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Vector Backbone Description: Vector Backbone:pK19mob; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_68382 Copy
Vector Backbone Description: Backbone Marker:epicentre; Vector Backbone:pCC1FOS; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_68380 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:26350500
Comments: Derived from Lajoie, et. al., Genomically recoded organisms expand biological functions, Science, 2013, with the following genomic modifications: ΔmutS:zeo, ΔtolC, Δbla:tolC, SerB-/ΔSerB
Proper citation: RRID:Addgene_68306 Copy
Species: Synthetic
Genetic Insert: S68TAG beta lactamase
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26350500
Comments: Used to assess phosphoserine or serine incorporation at TAG position corresponding to Ser68 in beta-lactamase protein. Ampicillin resistance only conferred if Ser is incorporated at this position.
Proper citation: RRID:Addgene_68305 Copy
Species: Homo sapiens
Genetic Insert: EXO1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21052091
Comments: Use BL21 cells for protein production.
EXO1b K589 was point-mutated to E, since this is the sequence described at UniProt (entry Q9UQ84). EXO1b also contains the following natural variants that were not corrected:
H354>R
E670>G
R723>C
Proper citation: RRID:Addgene_68269 Copy
Species: Homo sapiens
Genetic Insert: EXO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 HisC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15867354
Comments: EXO1b K589 was point-mutated to E, since this is the sequence described at UniProt (entry Q9UQ84). EXO1b also contains the following natural variants that were not corrected:
H354>R
E670>G
R723>C
Proper citation: RRID:Addgene_68268 Copy
Species: Homo sapiens
Genetic Insert: MBP-MEK1 S218TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes MBP-MEK1 (human) fusion protein, with TAG at position 218 and Ser encoded at position 222 in the activation loop.
Proper citation: RRID:Addgene_68301 Copy
Vector Backbone Description: Vector Backbone:pK19mobsacB; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_68389 Copy
Vector Backbone Description: Vector Backbone:pK19mobsacB; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_68388 Copy
Species: Synthetic
Genetic Insert: SpsCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68319 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS(4A) mCherry
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68275 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS mCherry
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68274 Copy
Species: Homo sapiens
Genetic Insert: PLK1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use BL21 cells for protein production
Proper citation: RRID:Addgene_68272 Copy
Species: Homo sapiens
Genetic Insert: PLK1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use BL21 cells for protein production
Proper citation: RRID:Addgene_68271 Copy
Species: Homo sapiens
Genetic Insert: AurA
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16158051
Comments: Use BL21 cells for protein production.
AurA mutation V57I has been described as “common coding region polymorphism” (Egan K et al., 2004, Carcinogenesis 25:2149)
Proper citation: RRID:Addgene_68270 Copy
Species: Synthetic
Genetic Insert: CdCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68314 Copy
Vector Backbone Description: Vector Backbone:pWB.attB; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23723152
Proper citation: RRID:Addgene_68313 Copy
Species: Synthetic
Genetic Insert: Ste7DS NLS mCitrine
Vector Backbone Description: Vector Backbone:pRS303; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26231587
Proper citation: RRID:Addgene_68278 Copy
Species: Synthetic
Genetic Insert: AzoCas9
Vector Backbone Description: Vector Backbone:pDREAM; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68329 Copy
Species: Synthetic
Genetic Insert: SepRS9/EF-Sep21, 1xtRNA-Sep
Vector Backbone Description: Vector Backbone:pKD; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26350500
Comments: See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.
Proper citation: RRID:Addgene_68284 Copy
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