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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
NEK5 gRNA (BRDN0001148037)
 
Resource Report
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RRID:Addgene_76120 NEK5 Homo sapiens Ampicillin PMID:26780180 The gRNA was designed to target the following Genbank reference sequence(s): NM_199289.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information. Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:31 0
MAST1 gRNA (BRDN0001149113)
 
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RRID:Addgene_76121 MAST1 Homo sapiens Ampicillin PMID:26780180 The gRNA was designed to target the following Genbank reference sequence(s): NM_014975.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information. Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:31 0
NEK1 gRNA (BRDN0001145167)
 
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RRID:Addgene_76128 NEK1 Homo sapiens Ampicillin PMID:26780180 The gRNA was designed to target the following Genbank reference sequence(s): NM_001199397.1,NM_001199398.1,NM_001199399.1,NM_001199400.1,NM_012224.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information. Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:32 0
MYT1 gRNA (BRDN0001147389)
 
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RRID:Addgene_76129 MYT1 Homo sapiens Ampicillin PMID:26780180 The gRNA was designed to target the following Genbank reference sequence(s): NM_004535.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information. Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:10:31 0
pDGB1omega2_SF (GB0466)
 
Resource Report
Resource Website
RRID:Addgene_68243 Solanum lycopersicum intergenic region Other Spectinomycin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1_omega2; Vector Types:Synthetic Biology, Unspecified; Bacterial Resistance:Spectinomycin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0
pDGB1omega1_SF (GB0158)
 
Resource Report
Resource Website
RRID:Addgene_68242 Solanum lycopersicum intergenic region Other Spectinomycin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1_omega1; Vector Types:Synthetic Biology, Unspecified; Bacterial Resistance:Spectinomycin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0
pDGB3_omega2R
 
Resource Report
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RRID:Addgene_68241 empty vector Other Spectinomycin PMID:23669743 Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega2R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:14 0
pDGB3_omega1R
 
Resource Report
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RRID:Addgene_68240 empty vector Other Spectinomycin PMID:23669743 Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3_omega1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:14 0
pICSL11057
 
Resource Report
Resource Website
RRID:Addgene_68253 Promote:TaU6+sgRNA HvPM19_1 Triticum aestivum (wheat) and synthetic Ampicillin PMID:26616834 Target seq: GCTCTCCACTCTGGGCTCTT Vector Backbone:pICH47761 (AddGene #48003); Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:09:14 0
pICSL11055
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_68252 Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos Cauliflower Mosaic Virus, Tobacco Mosaic Virus, Escherichia coli and Agrobacterium tumefaciens Ampicillin PMID:26616834 Vector Backbone:pICH47732 (AddGene #48000); Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:09:14 2
pICSL80036
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_68259 Coding sequence, hygromycin phophotransferase II (Escherichia coli) E. coli Spectinomycin PMID:26616834 Vector Backbone:pICH41308 AddGene #47998; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:15 1
pICSL12009
 
Resource Report
Resource Website
RRID:Addgene_68257 Promoter and 5UTR Ubiquitin (Zea mays) Zea mays Spectinomycin PMID:26616834 Vector Backbone:pICH41295 AddGene #47997; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:15 0
pICSL11062
 
Resource Report
Resource Website
RRID:Addgene_68256 Promoter_AtU6-26 + sgRNA_BolC.GA4a-2 Arabidopsis thaliana Ampicillin PMID:26616834 Target sequence: GTTGAGAGGGGAGCCGGTGA Vector Backbone:pICH47761 (AddGene #48003); Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:09:15 0
pICSL90002
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_68261 Promoter: U6-26 (Arabidopsis thaliana) Arabidopsis thaliana Spectinomycin PMID:26616834 Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:15 6
pICSL80037
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_68260 Coding sequence, neomycin phophotransferase II (Escherichia coli) E. coli Spectinomycin PMID:26616834 Vector Backbone:pICH41308 AddGene #47998; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-29 01:09:15 1
0.22 hA33 luciferase
 
Resource Report
Resource Website
RRID:Addgene_68157 A33 Homo sapiens Ampicillin PMID:12114523 Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin starts at -222 relative to ATG 2026-08-29 01:09:14 0
pPAt2S3 (GB0029)
 
Resource Report
Resource Website
RRID:Addgene_68162 At2S3 promoter Arabidopsis thaliana Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0
pHygromycin (GB0211)
 
Resource Report
Resource Website
RRID:Addgene_68204 Hygromycin resistance gene coding region Other Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0
pGUS (GB0208)
 
Resource Report
Resource Website
RRID:Addgene_68203 GUS coding region Other Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0
pPTctp (GB0083)
 
Resource Report
Resource Website
RRID:Addgene_68169 SlTCTP promoter Other Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-29 01:09:14 0

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