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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pYPQ134B2.0
 
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RRID:Addgene_167158 Tetracycline PMID:34168320 Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline 2026-08-15 01:09:41 0
∆DUSP6
 
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1+ mentions
RRID:Addgene_27977 DUSP6 Homo sapiens Tetracycline PMID:23839489 Catalytically inactive DUSP6 Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline C293S: mutation in the phosphatase domain 2026-08-15 01:13:09 1
DUSP6
 
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1+ mentions
RRID:Addgene_27975 DUSP6 Homo sapiens Tetracycline PMID:23839489 Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline 2026-08-15 01:13:09 4
pLEX SC4MOL 519
 
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RRID:Addgene_27984 SC4MOL Homo sapiens Tetracycline H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510 Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline H173Q mutation in SC4MOL 2026-08-15 01:13:08 0
pLEX SC4MOL
 
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RRID:Addgene_27983 SC4MOL Homo sapiens Tetracycline Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline 2026-08-15 01:13:09 0
pTGV-Express-Tet SMR5918
 
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RRID:Addgene_28035 Tetracycline PMID:11483365 Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 4286 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function. Backbone Size:8493; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline 2026-08-15 01:13:10 0
pNH10
 
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RRID:Addgene_40861 repeat NH10 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH6
 
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RRID:Addgene_40857 repeat NH6 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH4
 
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RRID:Addgene_40855 repeat NH4 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH2
 
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RRID:Addgene_40853 repeat NH2 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pNH8
 
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RRID:Addgene_40859 repeat NH8 Xanthomonas oryzae Tetracycline PMID:21493687 Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:39 0
pPROBE-TT
 
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1+ mentions
RRID:Addgene_37822 Promotorless gfp reporter gene bacteria Tetracycline PMID:11059491 Please refer to the attached table for a complete list of restriction sites in the MCS. This plasmid has the PstI site. Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:17 1
pPROBE-TT'
 
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RRID:Addgene_37823 Promotorless gfp reporter gene bacteria Tetracycline PMID:11059491 Please refer to the attached table for a complete list of restriction sites in the MCS. This plasmid has the PstI site. Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Tetracycline 2026-08-15 01:14:17 0
pCM157
 
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1+ mentions
RRID:Addgene_45863 cre recombinase Enterobacteria phage P1 Tetracycline PMID:12449384 The 1.1-kb XbaI-EcoRI fragment from pJW168 (PMID 9858684) was cloned between the XbaI and EcoRI sites of pCM62 (Addgene plasmid 45826) to generate the tetracycline-resistance conferring cre expression plasmid. Used with allelic exhange vectors--Addgene plasmids 46012 or 46013--to generate unmarked mutant strains in a broad bacterial host range. Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:6878; Vector Backbone:pCM62; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Tetracycline 2026-08-15 01:15:27 2
pCM130
 
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1+ mentions
RRID:Addgene_45828 Tetracycline PMID:11495985 XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity. Backbone Marker:Lidstrom Lab (Addgene plasmid 45826); Backbone Size:7965; Vector Backbone:pCM62; Vector Types:low-background broad-host-range promoter-probe vector using xylE as a reporter gene; Bacterial Resistance:Tetracycline 2026-08-15 01:15:26 3
pOSIP-TT (TetR, P21)
 
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RRID:Addgene_45989 Tetracycline PMID:24050148 Reference: St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j Backbone Size:7395; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:15:28 0
pKNOCK-Tc
 
Resource Report
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1+ mentions
RRID:Addgene_46259 Tetracycline PMID:10337469 Vector Backbone:original; Vector Types:Bacterial targeting; Bacterial Resistance:Tetracycline 2026-08-15 01:15:30 2
pINIT_tet
 
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RRID:Addgene_46974 Tetracycline PMID:21410291 Backbone Marker:unknown; Backbone Size:5595; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Tetracycline 2026-08-15 01:15:37 0
pDMS197
 
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RRID:Addgene_43831 Tetracycline PMID:9511756 The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination. Plasmid pRE107 (Addgene plasmid #43829) was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis. To use this plasmid with bla fusions and increase the functionality of this system, the depositing laboratory replaced the ApR gene as follows. The ApR gene flanked by the remaining BamHI sites was removed and replaced with either CmR, TcR or KmR resistance markers (see associated schematic image). The TcR gene was cloned on a blunted EcoRI–StyI fragment from pBR322 into the blunted BamHI sites to give pDMS197. The BamHI site near the MCS was regenerated after cloning; the BamHI site near the 5' end of TcR/TetR was not. The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Moreover, the use of Tc also allows an alternative negative selection using fusaric acid and chlortetracycline as described previously (Maloy and Nunn, 1981). Backbone Marker:Addgene plasmid 43829; Backbone Size:5173; Vector Backbone:pRE107 (modified from pGP704); Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Tetracycline 2026-08-15 01:15:10 0
pMflET-o4
 
Resource Report
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1+ mentions
RRID:Addgene_101314 oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) Mesoplasma florum Tetracycline PMID:28115382 Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:00:19 1

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