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Species: Homo sapiens
Genetic Insert: GRP78/BiP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11375416
Comments: The D238G point mutation found in the Addgene sequence is a known variation in human GRP78 that does not alter its chaperone or ATPase activities.
Proper citation: RRID:Addgene_32701 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15596010
Proper citation: RRID:Addgene_32707 Copy
Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32828 Copy
Genetic Insert: emGFP-miRNA
Vector Backbone Description: Backbone Marker:Richard O. Hynes group; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22138822
Proper citation: RRID:Addgene_32704 Copy
Species: Mus musculus
Genetic Insert: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1/Hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11457730
Proper citation: RRID:Addgene_32705 Copy
Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Backbone Marker:Guan lab; Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32826 Copy
Species: Homo sapiens
Genetic Insert: EAAT1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32813 Copy
Species: Homo sapiens
Genetic Insert: EAAT5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32817 Copy
Species: Homo sapiens
Genetic Insert: hEAAT2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32814 Copy
Genetic Insert: non-specific hairpin
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11315; Vector Backbone:pLemiR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21753754
Proper citation: RRID:Addgene_32809 Copy
Species: Homo sapiens
Genetic Insert: RIAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pcDNA4/HisMAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32803 Copy
Species: Homo sapiens
Genetic Insert: PANK1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3SMP. http://www.thesgc.org/structures/3SMP/
Proper citation: RRID:Addgene_32871 Copy
Species: Rattus norvegicus
Genetic Insert: μ2 subunit of clathrin adaptor complex AP-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10228163
Comments: NB: The HA tag (YPYDVPDYALE) was inserted within the protein between residues 236 and 237.
Proper citation: RRID:Addgene_32754 Copy
Species: Homo sapiens
Genetic Insert: EGFR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4672; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9857032
Comments: To create the EGFR-GFP fusion (in-frame), the the full-length cDNA of EGFR including 3' and 5' untranslated regions was cloned into pEGFP-N1 as an XbaI/HindIII fragment. This is EGFR-wt. The 3' end of the EGFR cDNA including STOP codon and untranslated region was removed by PCR and digestion. The 3' primer generated a new SstII (SacII) site.
Proper citation: RRID:Addgene_32751 Copy
Species: Rattus norvegicus
Genetic Insert: μ2 subunit of clathrin adaptor complex AP-2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10228163
Comments: NB: The HA tag (YPYDVPDYALE) was inserted within the protein, between residues 236 and 237.
Proper citation: RRID:Addgene_32752 Copy
Species: Homo sapiens
Genetic Insert: HECTD1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2LC3. http://www.thesgc.org/structures/2LC3/
Proper citation: RRID:Addgene_32860 Copy
Species: Homo sapiens
Genetic Insert: SAFB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12660241
Proper citation: RRID:Addgene_32742 Copy
Species: Toxoplasma gondii
Genetic Insert: MAPK2
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3RP9. http://www.thesgc.org/structures/3RP9/
Proper citation: RRID:Addgene_32862 Copy
Species: Homo sapiens
Genetic Insert: SETD1A
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3S8S. http://www.thesgc.org/structures/3S8S/
Proper citation: RRID:Addgene_32868 Copy
Species: Homo sapiens
Genetic Insert: TPK1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3S4Y. http://www.thesgc.org/structures/3S4Y/
Proper citation: RRID:Addgene_32865 Copy
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