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http://www.addgene.org/32686

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2720; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21418658

Proper citation: RRID:Addgene_32686 Copy   

•   Source: Addgene
  

http://www.addgene.org/32601

Genetic Insert: GPI-GFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604528

Proper citation: RRID:Addgene_32601 Copy   

•   Source: Addgene
  

http://www.addgene.org/32569

Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:7469; Vector Backbone:pTYF; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19212426
Comments: This plasmid grows quite slowly. Please allow up to two days for growth.

Proper citation: RRID:Addgene_32569 Copy   

•   Source: Addgene
  

http://www.addgene.org/32602

Genetic Insert: myr-Venus
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16604528
Comments: To generate myristoylated fluorescent fusion proteins, an N-terminal myristoylation tag derived from Src was generated by adding the sequences MGSSKSKPK to the N-terminus of any given fluorescent protein. This was achieved by using the following oligonucleotide: 5′ GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG). The GFP and Venus coding sequences were amplified from pEGFP-N1 (BD Biosciences, San Jose, CA) and pCS2-Venus (Nagai et al., 2002) to generate myr-GFP and myr-Venus, respectively, by high-fidelity polymerase chain reaction (PCR) using Pfx Polymerase (Invitrogen, La Jolla, CA) with the 5′ myristoylation primer combined with a 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC) primer, respectively. The resulting product was cloned into the EcoRI site of pCAGGS to generate pCX::myr-EGFP and pCX::myr-Venus

Proper citation: RRID:Addgene_32602 Copy   

•   Source: Addgene
  

http://www.addgene.org/32674

Vector Backbone Description: Backbone Size:3500; Vector Backbone:pISce1; Vector Types:Zebrafish Transgenesis; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_32674 Copy   

•   Source: Addgene
  

http://www.addgene.org/32669

Species: Mus musculus
Genetic Insert: Kir2.1-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812

Proper citation: RRID:Addgene_32669 Copy   

•   Source: Addgene
  

http://www.addgene.org/32650

Genetic Insert: FLPo
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879

Proper citation: RRID:Addgene_32650 Copy   

•   Source: Addgene
  

http://www.addgene.org/32641

Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.

Proper citation: RRID:Addgene_32641 Copy   

•   Source: Addgene
  

http://www.addgene.org/32642

Species: Mus musculus
Genetic Insert: tetO-Kir2.1-IRES-TLZ
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15157418
Comments: Addgene's sequencing results identified a single nucleotide a single nucleotide deletion at bp#1135 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results.

Proper citation: RRID:Addgene_32642 Copy   

•   Source: Addgene
  

http://www.addgene.org/32648

Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879

Proper citation: RRID:Addgene_32648 Copy   

•   Source: Addgene
  

http://www.addgene.org/32646

Genetic Insert: ChR2
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879

Proper citation: RRID:Addgene_32646 Copy   

•   Source: Addgene
  

http://www.addgene.org/32649

Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879

Proper citation: RRID:Addgene_32649 Copy   

•   Source: Addgene
  

http://www.addgene.org/32594

Genetic Insert: ECFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812

Proper citation: RRID:Addgene_32594 Copy   

•   Source: Addgene
  

http://www.addgene.org/32599

Genetic Insert: H2B-EGFP
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15619330
Comments: Please note that the plasmid contains a duplication of the H2B-EGFP region as compared to the original depositor sequence. The depositor has confirmed that the plasmid functions as described in the associated publication. The coding sequence for the human histone H2B gene (X57127) was amplified from genomic DNA by PCR using Pfx Polymerase (Invitrogen). The resulting product was cloned into pCR4 TOPO (Invitrogen) to generate pH2B. The H2B fragment was then cloned into plasmids pEGFP-N1, pDsRed2-N1 pDsRedExpress-N1 (BD Biosciences, Inc) in order to generate plasmids pH2B-EGFP, pH2B-DsRed2 and pH2B-DsRedExpress (oligonucleotide sequences are available upon request). The resulting fusions were then re-amplified by PCR and cloned into the XhoI site of pCAGGS [19] to generate pCX-H2B-EGFP, pCX-H2B-DsRed2 and pCX-H2B-DsRedExpress.

Proper citation: RRID:Addgene_32599 Copy   

•   Source: Addgene
  

http://www.addgene.org/32597

Genetic Insert: ECFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12079497

Proper citation: RRID:Addgene_32597 Copy   

•   Source: Addgene
  

http://www.addgene.org/32631

Species: Rabies virus
Genetic Insert: SADB19P
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879

Proper citation: RRID:Addgene_32631 Copy   

•   Source: Addgene
  

http://www.addgene.org/32591

Genetic Insert: IRES-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: BMC Biotechnol. 2006 Jan 12;6:4. Development of a new bicistronic retroviral vector with strong IRES activity. Martin P, Albagli O, Poggi MC, Boulukos KE, Pognonec P. Source CNRS UMR6548, Parc Valrose, Université de Nice Sophia Antipolis, Nice, France. [email protected]

Proper citation: RRID:Addgene_32591 Copy   

•   Source: Addgene
  

http://www.addgene.org/32636

Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: The genes which replaced GFP in the pcDNA-SADdeltaG-GFP plasmid were amplified by PCR using plasmids encoding mCherry and primer pairs with BamHI and NheI/NotI sequences at the ends.

Proper citation: RRID:Addgene_32636 Copy   

•   Source: Addgene
  

http://www.addgene.org/32637

Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: The genes which replaced GFP in the pcDNA-SADdeltaG-GFP plasmid were amplified by PCR using plasmids encoding mCherry and primer pairs with BamHI and NheI/NotI sequences at the ends.

Proper citation: RRID:Addgene_32637 Copy   

•   Source: Addgene
  

http://www.addgene.org/32634

Species: Rabies virus
Genetic Insert: B19N, B19P, B19M and B19L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences. The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.

Proper citation: RRID:Addgene_32634 Copy   

•   Source: Addgene