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Plasmids are provided by Addgene and DGRC.

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On page 269 showing 5361 ~ 5380 out of 744,118 results
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  • RRID:Addgene_117791

    This resource has 1+ mentions.

http://www.addgene.org/117791

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117791 Copy   


http://www.addgene.org/117793

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117793 Copy   


  • RRID:Addgene_117716

http://www.addgene.org/117716

Species: Oryza sativa
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4094; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized NLS-TIR1 is described in: Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).

Proper citation: RRID:Addgene_117716 Copy   


http://www.addgene.org/117718

Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4085; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized A. thaliana AID (IAA17) is described in: Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).

Proper citation: RRID:Addgene_117718 Copy   


  • RRID:Addgene_117717

    This resource has 1+ mentions.

http://www.addgene.org/117717

Species: Oryza sativa
Genetic Insert: TRANSPORT INHIBITOR RESPONSE 1
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4089; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized TIR1 is described in: Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).

Proper citation: RRID:Addgene_117717 Copy   


http://www.addgene.org/117714

Species: Synthetic
Genetic Insert: mVenus-mAID
Vector Backbone Description: Backbone Size:5482; Vector Backbone:pIREShygro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in: Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).

Proper citation: RRID:Addgene_117714 Copy   


http://www.addgene.org/117713

Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4850; Vector Backbone:pCDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in: Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).

Proper citation: RRID:Addgene_117713 Copy   


http://www.addgene.org/117784

Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117784 Copy   


http://www.addgene.org/117786

Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117786 Copy   


http://www.addgene.org/117781

Species: Danio rerio
Genetic Insert: gal4/VP16
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at . gal4-VP16 should be used in conjunction with a UAS expression line. May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117781 Copy   


  • RRID:Addgene_11772

http://www.addgene.org/11772

Species: Homo sapiens
Genetic Insert: WIP
Vector Backbone Description: Backbone Marker:Liu et al (see Addgene's vector database for details); Backbone Size:6000; Vector Backbone:pMX-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16606694

Proper citation: RRID:Addgene_11772 Copy   


  • RRID:Addgene_117701

    This resource has 1+ mentions.

http://www.addgene.org/117701

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117701 Copy   


  • RRID:Addgene_117789

    This resource has 1+ mentions.

http://www.addgene.org/117789

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117789 Copy   


  • RRID:Addgene_117700

    This resource has 1+ mentions.

http://www.addgene.org/117700

Species: Streptococcus canis
Genetic Insert: Streptococcus canis Cas9
Vector Backbone Description: Backbone Marker:Oxford Genetics; Vector Backbone:OG3569; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30397647

Proper citation: RRID:Addgene_117700 Copy   


http://www.addgene.org/117788

Species: Danio rerio
Genetic Insert: mRFP
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117788 Copy   


  • RRID:Addgene_117702

http://www.addgene.org/117702

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117702 Copy   


  • RRID:Addgene_117709

http://www.addgene.org/117709

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117709 Copy   


  • RRID:Addgene_117708

http://www.addgene.org/117708

Species: Mus musculus
Genetic Insert: Sod1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_117708 Copy   


  • RRID:Addgene_117790

    This resource has 1+ mentions.

http://www.addgene.org/117790

Species: Danio rerio
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32412410
Comments: Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at . May work in additional species beyond those listed. Please note that some discrepancies were found between Addgene's quality control result and the depositor's provided sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_117790 Copy   


  • RRID:Addgene_11779

    This resource has 1+ mentions.

http://www.addgene.org/11779

Genetic Insert: CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
Vector Backbone Description: Backbone Size:12859; Vector Backbone:None; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432520
Comments: Transgenes can be expressed from any RNA Pol II promoter as part of bicistronic unit comprising the KRAB-based repressor; tetO sequences are inserted into the vector LTR. Tet-on and Tet-off versions rely on repressors that bind in the absence or the presence of doxycycline, respectively. Addition of Pol III promoter-small hairpin RNA cassette allows for drug-controllable RNA interference (Tet-on shRNA). Cloning shRNA into pLVET, pLVCT, and pLVPT vectors: first clone shRNA into pLVTHM downstream of the tetO-H1 region. Then cut pLVTHM containing your shRNA with MscI-FspI and clone the insert containing the 3'LTR to target plasmid opened with MscI-FspI. FspI cuts into AmpR. This means for inverted clones AmpR will not be restored; after selection you will be left with clones with the shRNA cassette and functional AmpR. pLVTHM and packaging plasmid for this system are also available at Addgene http://www.addgene.org/rnaitools Please visit Trono lab website http://tronolab.epfl.ch to see frequently asked questions on cloning strategies and packaging.

Proper citation: RRID:Addgene_11779 Copy   



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