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Genetic Insert: pac-P2A-mTFP-Jumblelw1
Vector Backbone Description: Vector Backbone:NK676; Vector Types:Choanoflagellate expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120856 Copy
Species: Homo sapiens
Genetic Insert: HDM2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10980695
Proper citation: RRID:Addgene_12087 Copy
Genetic Insert: F' Tra operon
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16141338
Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.
Proper citation: RRID:Addgene_12082 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5mARF17
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGreenII0129; Vector Types:Plant Expression, plant transformation via Agrobacterium; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15829600
Comments: See "sequence" for full insert sequence. See article for cloning details.
Proper citation: RRID:Addgene_12081 Copy
Genetic Insert: NS5 ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7735; Vector Backbone:pLenti6.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120843 Copy
Genetic Insert: NS4B ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7735; Vector Backbone:pLenti6.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120840 Copy
Genetic Insert: ZIKV NS5-eYFP "NLS-mut"
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120832 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120833 Copy
Genetic Insert: Capsid ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7735; Vector Backbone:pLenti6.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120834 Copy
Genetic Insert: pr-Membrane ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7735; Vector Backbone:pLenti6.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_120835 Copy
Genetic Insert: InsDown_GSY-NotI
Vector Backbone Description: Vector Backbone:pCR4Blunt-TOPO; Vector Types:Cloning/assembly plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31148366
Proper citation: RRID:Addgene_120791 Copy
Genetic Insert: InsDown-Mfe
Vector Backbone Description: Vector Backbone:pCR4Blunt-TOPO; Vector Types:Cloning/assembly plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31148366
Proper citation: RRID:Addgene_120792 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16248887
Comments: It targets gamma2 GABAA receptor subunit mRNA, nucleotides 130–155 located in the protein coding region of GenBank accesion number L08497. It targets both gamma2S and gamma2L mRNAs).
Proper citation: RRID:Addgene_120794 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16248887
Comments: 3-point mutations (negative control for shRNA2 targeting gamma2 mRNA, plasmid 120794).
Proper citation: RRID:Addgene_120795 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16248887
Comments: It targets gamma2 GABAA receptor subunit mRNA, nucleotides 1467–1491 located in the 3'-UTR of GenBank accesion number L08497. It targets both gamma2S and gamma2L mRNAs.
Proper citation: RRID:Addgene_120796 Copy
Genetic Insert: ZIKV NS5-eYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120831 Copy
Genetic Insert: Envelope ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120821 Copy
Genetic Insert: NS3 ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120822 Copy
Genetic Insert: NS1 ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120824 Copy
Genetic Insert: NS5 ZIKV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5535; Vector Backbone:pcDNA3.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29572905
Proper citation: RRID:Addgene_120825 Copy
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