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Species: Synthetic
Genetic Insert: sgRNA1-boxB-MS2
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27088723
Comments: Want all of the CRISPRainbow constructs? Request the set here: https://www.addgene.org/kits/pederson-crisprainbow/
Proper citation: RRID:Addgene_75396 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
Vector Backbone Description: Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Backbone Size:3055; Vector Backbone:pUPD; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75399 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pAAV-D(+)-U6-siRNA-CMV-GFP; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26075822
Proper citation: RRID:Addgene_75438 Copy
Species: Rattus norvegicus
Genetic Insert: snca
Vector Backbone Description: Vector Backbone:pAAV-D(+)-U6-siRNA-CMV-GFP; Vector Types:Mammalian Expression, AAV, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26075822
Proper citation: RRID:Addgene_75437 Copy
Species: Homo sapiens
Genetic Insert: TTK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001166691.1,NM_003318.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75580 Copy
Species: Homo sapiens
Genetic Insert: TNK2
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001010938.1,NM_005781.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75583 Copy
Species: Homo sapiens
Genetic Insert: TTK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001166691.1,NM_003318.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75581 Copy
Species: Homo sapiens
Genetic Insert: AKT1
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001014431.1,NM_001014432.1,NM_005163.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75500 Copy
Species: Homo sapiens
Genetic Insert: CSNK2A2
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001896.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75586 Copy
Species: Homo sapiens
Genetic Insert: TNK2
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001010938.1,NM_005781.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75585 Copy
Species: Homo sapiens
Genetic Insert: BCKDK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001122957.2,NM_001271926.1,NM_005881.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75504 Copy
Species: Homo sapiens
Genetic Insert: AKT1
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001014431.1,NM_001014432.1,NM_005163.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75502 Copy
Species: Homo sapiens
Genetic Insert: EPHA1
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_005232.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75589 Copy
Species: Homo sapiens
Genetic Insert: INSR
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target a Genbank reference sequence which has since been removed from the database. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75507 Copy
Species: Homo sapiens
Genetic Insert: INSR
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target a Genbank reference sequence which has since been removed from the database. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75506 Copy
Species: Homo sapiens
Genetic Insert: BCKDK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001122957.2,NM_001271926.1,NM_005881.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75505 Copy
Species: Homo sapiens
Genetic Insert: MAP3K2
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_006609.4. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75509 Copy
Species: Homo sapiens
Genetic Insert: PBK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001278945.1,NM_018492.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75573 Copy
Species: Homo sapiens
Genetic Insert: NUAK1
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_014840.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75576 Copy
Species: Homo sapiens
Genetic Insert: PBK
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: The gRNA was designed to target the following Genbank reference sequence(s): NM_001278945.1,NM_018492.3. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_75574 Copy
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