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Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101763 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔlamB ΔompC
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102263 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompC ΔompF
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102265 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔompF
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102262 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4378; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101854 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4835; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101855 Copy
Species: Mus musculus
Genetic Insert: Ascl1, Brn2
Vector Backbone Description: Backbone Size:5845; Vector Backbone:pUC19; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646119
Proper citation: RRID:Addgene_101852 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101866 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101867 Copy
Species: Mus musculus
Genetic Insert: Mds1-Evi-1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101860 Copy
Species: NA
Genetic Insert: Kanamycin casette (in place of synpcc7942_2333-2335 operon)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101839 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431A T432A with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101837 Copy
Species: Homo sapiens
Genetic Insert: Flag-BPTF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6].
Proper citation: RRID:Addgene_102242 Copy
Species: Homo sapiens
Genetic Insert: dCas9-HA-BFP-KRAB-NLS
Vector Backbone Description: Backbone Size:9651; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein.
Proper citation: RRID:Addgene_102244 Copy
Species: S. elongatus PCC7942
Genetic Insert: RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101831 Copy
Species: NA
Genetic Insert: Kanamycin casette (in place of glgC gene)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101842 Copy
Species: E. coli
Genetic Insert: Ptrc promoter driving GalP transporter expression
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101843 Copy
Species: NA
Genetic Insert: Kan casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101844 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102256 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102257 Copy
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