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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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FKBP_SCF2_LD6_NTEV Resource Report Resource Website |
RRID:Addgene_58878 | MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease | Synthetic | Ampicillin | PMID:24611683 | Backbone Size:5521; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:19 | 0 | ||
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CD4_LD 0_TEV Resource Report Resource Website |
RRID:Addgene_58870 | MESA protease chain with CD4 ectodomain and TEV protease | Synthetic | Ampicillin | PMID:24611683 | Backbone Size:5520; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:19 | 0 | ||
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PX543 SpCas9(del175-307) Resource Report Resource Website |
RRID:Addgene_58888 | hSpCas9 | Synthetic | Ampicillin | PMID:24529477 | For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/. | Vector Backbone:PX165; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | del amino acids 175-307 | 2026-08-15 01:17:19 | 0 |
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PX544 SpCas9(del1098-1368) Resource Report Resource Website |
RRID:Addgene_58889 | hSpCas9 | Synthetic | Ampicillin | PMID:24529477 | For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/. | Vector Backbone:PX165; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | del amino acids 1098-1368 | 2026-08-15 01:17:19 | 0 |
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pcDNA-NES-ZapCY2 Resource Report Resource Website |
RRID:Addgene_59016 | NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor | Synthetic | Ampicillin | PMID:23992616 | Genetically encoded, ratiometric, fluorescent biosensor. Contains nuclear exclusion signal sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins. The Zn(II) binding domain of ZapCY2 is derived from ZapCY1. The first cysteine in each zinc finger is mutated to histidine. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | See comment | 2026-08-15 01:17:20 | 0 |
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pcDNA-NES-ZapCmR2 Resource Report Resource Website |
RRID:Addgene_59015 | NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor | Synthetic | Ampicillin | PMID:23173058 | Genetically encoded, ratiometric, fluorescent biosensor. Contains nuclear exclusion signal sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains Clover and mRuby fluorescent proteins. The Zn(II) binding domain of ZapCmR2 is derived from ZapCmR1.1. The first cysteine in the second zinc finger is mutated to histidine. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | See comment | 2026-08-15 01:17:20 | 0 |
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pcDNA-mito-ZifCV1.173 Resource Report Resource Website |
RRID:Addgene_59010 | mito-ZifCV1.173 genetically encoded Zn(II) sensor | Synthetic | Ampicillin | PMID:22850482 | Genetically encoded, ratiometric, fluorescent biosensor targeted to the mitochondria. mito-ZifCV1.173 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from mammalian Zif268. Contains truncated CFP and circularly permuted Venus 173. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:20 | 0 | |
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pcDNA-NLS-ZapCmR1.1 Resource Report Resource Website |
RRID:Addgene_59011 | NLS-ZapCmR1.1 genetically encoded Zn(II) sensor | Synthetic | Ampicillin | PMID:23173058 | Genetically encoded, ratiometric, fluorescent biosensor. Contains NLS sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains Clover and mRuby fluorescent proteins. The Zn(II) binding domain of ZapCmR1.1 is derived from ZapCY1. The first cysteine in the first zinc finger is mutated to histidine. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | See comment | 2026-08-15 01:17:20 | 0 |
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AAVS1-TALEN-R Resource Report Resource Website 50+ mentions |
RRID:Addgene_59026 | AAVS1-TALEN-R | Synthetic | Ampicillin | PMID:24931489 | Backbone Marker:Feng Zhang Lab; Backbone Size:7961; Vector Backbone:pTALEN; Vector Types:Mammalian Expression, AAV, TALEN; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:20 | 57 | ||
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pMitoLOC Resource Report Resource Website 1+ mentions |
RRID:Addgene_58980 | mCherry | Synthetic | Ampicillin | PMID:26184437 | Backbone Size:4872; Vector Backbone:pUG35; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:20 | 5 | ||
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pcDNA-mito-ZapCY1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_58996 | mito-ZapCY1 high affinity Zn(II) sensor | Synthetic | Ampicillin | PMID:22850482 | Genetically encoded, ratiometric, fluorescent biosensor. mito-ZapCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:20 | 2 | |
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pcDNA-mito-ZifCV1.49 Resource Report Resource Website |
RRID:Addgene_59009 | mito-ZifCV1.49 genetically encoded Zn(II) sensor | Synthetic | Ampicillin | PMID:22850482 | Genetically encoded, ratiometric, fluorescent biosensor targeted to the mitochondria. mito-ZifCV1.49 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence. Zn(II)-binding domain derived from mammalian Zif268. Contains truncated CFP and circularly permuted Venus 49. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:20 | 0 | |
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FCK-QuasAr2-Citrine Resource Report Resource Website 1+ mentions |
RRID:Addgene_59172 | QuasAr2-Citrine eFRET | Synthetic | Ampicillin | PMID:25118186 | Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:21 | 2 | ||
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FCK-QuasAr2-mRuby2 eFRET Resource Report Resource Website |
RRID:Addgene_59174 | QuasAr2-mRuby2 eFRET | Synthetic | Ampicillin | PMID:25118186 | Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:21 | 0 | ||
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p201B Cas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_59177 | Cas9 | Synthetic | Kanamycin | PMID:25879861 | Backbone Size:6200; Vector Backbone:pPZP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:21 | 1 | ||
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p201G Cas9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_59178 | Cas9 | Synthetic | Kanamycin | PMID:25879861 | Backbone Size:6200; Vector Backbone:pPZP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:21 | 3 | ||
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pT2SC Resource Report Resource Website 1+ mentions |
RRID:Addgene_59382 | TT-ISceI-cat | Synthetic | Chloramphenicol and Ampicillin | PMID:25255806 | iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. | Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:17:23 | 1 | |
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pT2ST Resource Report Resource Website |
RRID:Addgene_59384 | TT-ISceI-dfrA | Synthetic | Ampicillin and Trimethoprim | PMID:25255806 |
iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The trimethoprim resistance gene was taken from EZ-Tn5™ |
Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Trimethoprim | 2026-08-15 01:17:23 | 0 | |
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pT2SK Resource Report Resource Website |
RRID:Addgene_59383 | TT-ISceI-kan | Synthetic | Ampicillin and Kanamycin | PMID:25255806 | iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The kanamycin resistance gene was taken from pACYC177. We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. | Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Ampicillin and Kanamycin | 2026-08-15 01:17:23 | 0 | |
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pT2SCb Resource Report Resource Website |
RRID:Addgene_59385 | TT-ISceI-cat2 | Synthetic | Chloramphenicol and Ampicillin | PMID:25255806 | iGEM parts BBa_B1002 and BBa_B1006 were used to build the double terminator element. The chloramphenicol resistance gene was taken from pACYC187. The 3'-end was modified from 5'-GGGCGGGGCGTAA to 5'-AGGAGGTGCATAA in order to provide a site resembling the consensus bacterial ribosomal binding site (5'-AGGAGGTAAATAA). We developed GetX (https://sourceforge.net/projects/getx/), a stand-alone python script that allows the user to design mutation cassettes for scarless genome editing in bacteria using our previously described two-step recombination method. Please see the document linked under the Resource Information heading above for additional information on installing and using the script. | Backbone Marker:Copley Lab; Backbone Size:1887; Vector Backbone:pHA1887, an 1887 bp fragment extending from bp 690 to bp 2576 of pUC19 (Acc. No. M77789); Vector Types:template; Bacterial Resistance:Chloramphenicol and Ampicillin | The 3’-end of the chloramphenicol resistance gene was modified to 5’-AGGAGGTGCATAA to create a sequence resembling the consensus bacterial ribosomal binding site (5’-AGGAGGTAAATAA). | 2026-08-15 01:17:23 | 0 |
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