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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)
 
Resource Report
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RRID:Addgene_14852 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-08-15 01:07:08 0
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)
 
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RRID:Addgene_14851 GAL1 promoter TATA-C2 Saccharomyces cerevisiae Ampicillin PMID:15166317 pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3’ to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA. Backbone Size:0; Vector Backbone:pJRL2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin TATA-C2. TATA box mutated from TATATAAG to TCTATAAG. 2026-08-15 01:07:08 0
pGBW-m4046459
 
Resource Report
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RRID:Addgene_148971 SARS-CoV-2 nsp10 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046567
 
Resource Report
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RRID:Addgene_148972 SARS-CoV-2 RNA-dependent RNA polymerase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:09 0
pGBW-m4046435
 
Resource Report
Resource Website
RRID:Addgene_148974 SARS-CoV-2 helicase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046380
 
Resource Report
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RRID:Addgene_148977 SARS-CoV-2 E (envelope) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:10 0
pGBW-m4046160
 
Resource Report
Resource Website
RRID:Addgene_148978 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:10 0
pGBW-m4046503
 
Resource Report
Resource Website
RRID:Addgene_149075 SARS-CoV-2 E (envelope) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724392.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:11 0
pGBW-m4046278
 
Resource Report
Resource Website
RRID:Addgene_149000 SARS-CoV-2 ORF6 protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724394.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:11 0
pGBW-m4046305
 
Resource Report
Resource Website
RRID:Addgene_149004 SARS-CoV-2 helicase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725308.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046454
 
Resource Report
Resource Website
RRID:Addgene_149006 SARS-CoV-2 RNA-dependent RNA polymerase Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725307.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:11 0
pGBW-m4046314
 
Resource Report
Resource Website
RRID:Addgene_149007 SARS-CoV-2 endoRNAse Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046239
 
Resource Report
Resource Website
RRID:Addgene_149009 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:11 0
pGBW-m4046296
 
Resource Report
Resource Website
RRID:Addgene_149012 SARS-CoV-2 ORF8 protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724396.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046510
 
Resource Report
Resource Website
RRID:Addgene_149014 SARS-CoV-2 nsp10 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725306.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:11 0
pGBW-m4046313
 
Resource Report
Resource Website
RRID:Addgene_149016 SARS-CoV-2 ORF3a protein Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724391.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:10 0
pGBW-m4046460
 
Resource Report
Resource Website
RRID:Addgene_149017 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:11 0
pGBW-m4046182
 
Resource Report
Resource Website
RRID:Addgene_149019 SARS-CoV-2 nsp4 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725300.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:10 0
pGBW-m4046375
 
Resource Report
Resource Website
RRID:Addgene_149030 SARS-CoV-2 nsp2 Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009725298.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:07:10 0
pGBW-m4046310
 
Resource Report
Resource Website
RRID:Addgene_149022 SARS-CoV-2 M (membrane) Saccharomyces cerevisiae Ampicillin NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:07:11 0

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