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Species: Homo sapiens
Genetic Insert: HDR-Enhancer domain of human CtIP protein
Vector Backbone Description: Backbone Marker:Church lab A; Vector Backbone:hCas9 Addgene ID 41815; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29556040
Comments: Note from depositor: The sequence used for the HDR enhancer domain of CtIP has several differences compared to the reference human CtIP cDNA sequence including T176A. These differences were present in the CtIP expression vector used to make the constructs and do not affect activity.
Proper citation: RRID:Addgene_109400 Copy
Species: Homo sapiens
Genetic Insert: CtIP
Vector Backbone Description: Backbone Marker:Church lab; Vector Backbone:hCas9 Addgene ID 41815; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29556040
Proper citation: RRID:Addgene_109403 Copy
Genetic Insert: Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16483934
Comments: The plasmid constructed by PCR cloning HCV IRES into the NheI site of pRL-TKx6 and inserting the Firefly luciferase ORF from pGL3 into the resulting vectorl.
Proper citation: RRID:Addgene_11510 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 46Q glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11515 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115144 Copy
Species: Synthetic
Genetic Insert: human codon optimized Mb3Cas12a
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene Plasmid #11179); Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115142 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115136 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA2 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115138 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA3.1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115139 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKR
Proper citation: RRID:Addgene_115120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1151 Copy
Species: Mus musculus
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28842570
Proper citation: RRID:Addgene_115341 Copy
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8724; Vector Backbone:pDest-590; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11519 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8266; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11518 Copy
Species: Mus musculus
Genetic Insert: shRNA targeting Plxnb1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29981480
Proper citation: RRID:Addgene_115174 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8932; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11520 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1153 Copy
Species: Homo sapiens
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Vector Backbone:pLX317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_115439 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Erg11-iGFPx3
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Proper citation: RRID:Addgene_115422 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: vps10-104
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30858194
Comments: Please note sequence is from JK93da yeast strain rather than S288C
Proper citation: RRID:Addgene_115426 Copy
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