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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145549 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64536; Backbone Size:4434; Vector Backbone:pRG206MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154821 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway middle entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32697972
Comments: Can be recombined with any p5E vector containing a promoter to allow for tissue-specific expression of QFGal4. Note that an SV40pA is included after QFGal4.
Proper citation: RRID:Addgene_155118 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: QFGal4
Vector Backbone Description: Backbone Size:4053; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Expression in zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32697972
Comments: Can be linearized with NotI for in vitro synthesis of QFGal4
Proper citation: RRID:Addgene_155126 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P2A-QFGal4
Vector Backbone Description: Backbone Marker:Karl J. Clark; Backbone Size:3310; Vector Backbone:pK; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32697972
Comments: pGTag series vectors were described in PMID: 32412410. pGTag-P2A-QFGal4-SV40 was modified from pGTag-eGFP-SV40 (Plasmid #117813).
Proper citation: RRID:Addgene_155127 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: α Galactosidase SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153504 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Invertase 2 SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prepro SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc/Ca = Saccharomyces cerevisiae / Candida albicans chimera (residues ScNM 1-40, CaNM 49-141, ScNM 124-254), NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8633.
Proper citation: RRID:Addgene_15600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sup35 NM
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pAED4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17495929
Comments: Protein expression inducible by IPTG. Sc = Saccharomyces cerevisiae, NM = N-terminal and Middle domains of Sup35.
Lindquist plasmid # 8631.
Proper citation: RRID:Addgene_15598 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNQ1
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 7250
Proper citation: RRID:Addgene_15596 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ENT2
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 8028
Proper citation: RRID:Addgene_15593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PAN1
Vector Backbone Description: Backbone Size:0; Vector Backbone:p316 CUP; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832049
Comments: Figure 5 in article.
Lindquist lab number: 8027
Proper citation: RRID:Addgene_15592 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP-gRNA
Vector Backbone Description: Backbone Size:1861; Vector Backbone:pTPGI; Vector Types:Insert storage (replicative in E. coli); Bacterial Resistance:Ampicillin
Defining Citation: PMID:33191917
Proper citation: RRID:Addgene_157656 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P-rhaP-BAD-malE-His tag-smt3-eGFP
Vector Backbone Description: Vector Backbone:pTST101 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19127343
Proper citation: RRID:Addgene_135085 Copy
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