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Species: Mus musculus
Genetic Insert: NMHC II-C
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14594953
Proper citation: RRID:Addgene_10843 Copy
Species: Synthetic
Genetic Insert: zArchon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pTol2-UAS; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108427 Copy
Species: Synthetic
Genetic Insert: attB-eGFP-4xGly-TEV-Flag-6xHis
Vector Backbone Description: Backbone Marker:Kenan Murphy; Backbone Size:3159; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating EGFP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108434 Copy
Species: Synthetic
Genetic Insert: TetR + PTet*-YFP
Vector Backbone Description: Vector Backbone:p15A-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30478458
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108529 Copy
Species: Synthetic
Genetic Insert: Telomere-targeting sgRNA
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108649 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: hyBRET-ERK-EV
Vector Backbone Description: Backbone Size:4739; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29895862
Proper citation: RRID:Addgene_108652 Copy
Species: Homo sapiens
Genetic Insert: HHR23A
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10373495
Comments: See author's map.
Proper citation: RRID:Addgene_10864 Copy
Species: Homo sapiens
Genetic Insert: RNASEH2B
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21177854
Proper citation: RRID:Addgene_108693 Copy
Species: Mus musculus
Genetic Insert: RANKL (receptor activator of nuclear factor kappa-B ligand)
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4970; Vector Backbone:pGEX4T2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17419679
Proper citation: RRID:Addgene_108571 Copy
Species: Synthetic
Genetic Insert: dSpyCas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29735996
Comments: CRISPR-Cas9 nuclear dynamics and target recognition in living cells. Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., and Pederson, T. (2016). CRISPR-Cas9 nuclear dynamics and target recognition in living cells. The Journal of Cell Biology 214, 529–537.
Proper citation: RRID:Addgene_108570 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4888; Vector Backbone:ColE1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33732871
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/13/264390 for bioRxiv preprint.
Proper citation: RRID:Addgene_108685 Copy
Species: Homo sapiens
Genetic Insert: RNASEH1 (nuclear isoform)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21245041
Proper citation: RRID:Addgene_108699 Copy
Species: Homo sapiens
Genetic Insert: RNASEH2B
Vector Backbone Description: Backbone Marker:LMBP; Backbone Size:4804; Vector Backbone:pEGFP-Dest (LMBP 4542); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21245041
Proper citation: RRID:Addgene_108697 Copy
Species: Synthetic
Genetic Insert: PURO
Vector Backbone Description: Backbone Marker:invitrogen; Vector Backbone:pENTR1A; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29330493
Proper citation: RRID:Addgene_108670 Copy
Species: Homo sapiens
Genetic Insert: RNASEH2A
Vector Backbone Description: Backbone Marker:LMBP; Backbone Size:4801; Vector Backbone:pEGFP-Dest (LMBP 4542); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21245041
Proper citation: RRID:Addgene_108700 Copy
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_108542 Copy
Vector Backbone Description: Vector Backbone:pMpGE010; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30379827
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/14/277350 for BioRxiv preprint
Proper citation: RRID:Addgene_108681 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1-UGI-LVA
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29403014
Proper citation: RRID:Addgene_108551 Copy
Genetic Insert: stuffer
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16564017
Comments: This is the recommended vector for cloning and expressing new shRNA sequences. This plasmid is being used by The RNAi Consortium to produce their shRNA library http://www.broad.mit.edu/genome_bio/trc/ The 1.9kb stuffer can be released with AgeI and EcoRI and replaced with your shRNA sequence of choice.
The link to the author's map shows the key features of this vector before the 1.9kb stuffer was cloned in. The link to the sequence shows the entire sequence with the stuffer sequence in capital letters.
Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_10878 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-p3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29621251
Proper citation: RRID:Addgene_108884 Copy
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