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http://www.addgene.org/21662

Species: Homo sapiens
Genetic Insert: Orai1
Vector Backbone Description: Backbone Size:7480; Vector Backbone:MO70; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17293345
Comments: See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).

Proper citation: RRID:Addgene_21662 Copy   

•   Source: Addgene

http://www.addgene.org/21664

Species: Homo sapiens
Genetic Insert: NFAT4
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10497131

Proper citation: RRID:Addgene_21664 Copy   

•   Source: Addgene

http://www.addgene.org/21635

Species: Homo sapiens
Genetic Insert: Atg14L
Vector Backbone Description: Backbone Size:4727; Vector Backbone:pEGFP-C2 (Clontech); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19270696

Proper citation: RRID:Addgene_21635 Copy   

•   Source: Addgene

http://www.addgene.org/21639

Species: Homo sapiens
Genetic Insert: IGF-II mRNA-binding protein 1
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation

Proper citation: RRID:Addgene_21639 Copy   

•   Source: Addgene

http://www.addgene.org/21753

Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337722

Proper citation: RRID:Addgene_21753 Copy   

•   Source: Addgene

http://www.addgene.org/21630

Species: Mus musculus
Genetic Insert: myelin protein zero intron1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536

Proper citation: RRID:Addgene_21630 Copy   

•   Source: Addgene

http://www.addgene.org/21751

Species: M. magnetotacticum
Genetic Insert: magA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11250081
Comments: The sequence inserted into the pCR2.1 vector is larger than just the magA gene. The magA gene is preceded by 373 bp of genomic DNA and followed by 30 bp of genomic DNA. The gene contains 2 amino acid substitutions - S94L and P390S. The cloned gene was used primarily as a probe. Also see Zurkiya et al. Magn Reson Med. 2008 Jun;59(6):1225-31.

Proper citation: RRID:Addgene_21751 Copy   

•   Source: Addgene

http://www.addgene.org/21645

Species: Homo sapiens
Genetic Insert: Cyclin D2 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.

Proper citation: RRID:Addgene_21645 Copy   

•   Source: Addgene

http://www.addgene.org/21649

Species: Homo sapiens
Genetic Insert: Dicer 1 long UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Long means that all functional alternative polyadenylation signals have been deleted.

Proper citation: RRID:Addgene_21649 Copy   

•   Source: Addgene

http://www.addgene.org/21644

Species: Homo sapiens
Genetic Insert: Cyclin D2 short UTR
Vector Backbone Description: Backbone Marker:Available from Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19703394
Comments: Short means always shortest 3’UTR due to alternative polyadenylation.

Proper citation: RRID:Addgene_21644 Copy   

•   Source: Addgene

http://www.addgene.org/21737

Species: Synthetic
Genetic Insert: Perceval (fluorescent ATP:ADP sensor)
Vector Backbone Description: Backbone Marker:British Biotech; Backbone Size:5076; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19122669

Proper citation: RRID:Addgene_21737 Copy   

•   Source: Addgene

http://www.addgene.org/21690

Species: Homo sapiens
Genetic Insert: Golgi Phosphoprotein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:897; Vector Backbone:pEF-DEST51; Vector Types:Mammalian Expression, Invitrogen Gateway Destination vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19553991

Proper citation: RRID:Addgene_21690 Copy   

•   Source: Addgene

http://www.addgene.org/21746

Species: Rattus norvegicus
Genetic Insert: mTOR (1271-2008)
Vector Backbone Description: Backbone Marker:BD Pharmingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12718876

Proper citation: RRID:Addgene_21746 Copy   

•   Source: Addgene

http://www.addgene.org/21744

Species: Drosophila melanogaster
Genetic Insert: mCherry::dMoeABD
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2931; Vector Backbone:pBS Sk-; Vector Types:bacterial propagation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19098902

Proper citation: RRID:Addgene_21744 Copy   

•   Source: Addgene

http://www.addgene.org/21627

Species: Homo sapiens
Genetic Insert: hOCT4 - 2A - hSOX2 - 2A - hKLF4
Vector Backbone Description: Backbone Size:9692; Vector Backbone:pDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19415770
Comments: Plasmid constructed by Kevin Pawlik

Proper citation: RRID:Addgene_21627 Copy   

•   Source: Addgene

http://www.addgene.org/21835

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Comments: The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34.

Proper citation: RRID:Addgene_21835 Copy   

•   Source: Addgene

http://www.addgene.org/21834

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086

Proper citation: RRID:Addgene_21834 Copy   

•   Source: Addgene

http://www.addgene.org/21833

Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V. FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21833 Copy   

•   Source: Addgene

http://www.addgene.org/21832

Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V. FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21832 Copy   

•   Source: Addgene

http://www.addgene.org/21842

Species: L. pneumophila
Genetic Insert: fabI
Vector Backbone Description: Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19578436

Proper citation: RRID:Addgene_21842 Copy   

•   Source: Addgene