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Species: Homo sapiens
Genetic Insert: 3' UTR FAM104A-202
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117345 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR FAM102A-203
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117344 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: shble
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Thraustochytrid expression; Bacterial Resistance:Ampicillin
Comments: cloning method is Infusion cloning (Clontech) and restriction digest
Proper citation: RRID:Addgene_117226 Copy
Species: Erwinia uredovora, Escherichia Coli, Agrobacterium aurantiacum
Genetic Insert: Canthaxanthin Operon
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pET28-b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31358887
Proper citation: RRID:Addgene_117225 Copy
Species: Streptoalloteichus hindustanus
Genetic Insert: shble
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Thraustochytrid expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32251396
Comments: cloning method is Infusion cloning (Clontech) and restriction digest
Proper citation: RRID:Addgene_117228 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR LEMD2-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117349 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR KIAA2013-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117348 Copy
Species: Homo sapiens
Genetic Insert: SMAD1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8653785
Proper citation: RRID:Addgene_11735 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8752209
Proper citation: RRID:Addgene_11734 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR TP73-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117333 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR ADGRL1-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117336 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR ASIC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117339 Copy
Species: Synthetic
Genetic Insert: gRNA miR-124-1-3'
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:9157; Vector Backbone:PX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: Cas9 with NLS included
Proper citation: RRID:Addgene_117324 Copy
Species: Synthetic
Genetic Insert: miR-124 sponge
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Proper citation: RRID:Addgene_117327 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR CTDSP1-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117328 Copy
Species: Mus musculus
Genetic Insert: partial sequence of mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30587510
Proper citation: RRID:Addgene_117274 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-CTRL
Vector Backbone Description: Backbone Marker:Church Lab; Backbone Size:7460; Vector Backbone:PB-TRE; Vector Types:Mammalian Expression, Transposon-mediated integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' BamHI cloning site not destroyed, 3' BamHI cloning site destroyed
Proper citation: RRID:Addgene_117319 Copy
Species: Mus musculus
Genetic Insert: Egr-1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15225550
Proper citation: RRID:Addgene_11729 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: Mus musculus
Genetic Insert: Sox9
Vector Backbone Description: Backbone Size:9063; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30127505
Proper citation: RRID:Addgene_117269 Copy
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