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http://www.addgene.org/101818

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101818 Copy   

•   Source: Addgene

http://www.addgene.org/101815

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101815 Copy   

•   Source: Addgene

http://www.addgene.org/101816

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101816 Copy   

•   Source: Addgene

http://www.addgene.org/101770

Species: Homo sapiens
Genetic Insert: DYRK1A
Vector Backbone Description: Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38966995

Proper citation: RRID:Addgene_101770 Copy   

•   Source: Addgene

http://www.addgene.org/101811

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101811 Copy   

•   Source: Addgene

http://www.addgene.org/101779

Species: Homo sapiens
Genetic Insert: BAF (BANF1)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28841419

Proper citation: RRID:Addgene_101779 Copy   

•   Source: Addgene

http://www.addgene.org/101774

Species: Homo sapiens
Genetic Insert: BAF (BANF1)
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:10370; Vector Backbone:Addgene plasmid # 52962; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28841419

Proper citation: RRID:Addgene_101774 Copy   

•   Source: Addgene

http://www.addgene.org/101708

Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2α1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29132331

Proper citation: RRID:Addgene_101708 Copy   

•   Source: Addgene

http://www.addgene.org/101709

Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29132331

Proper citation: RRID:Addgene_101709 Copy   

•   Source: Addgene

http://www.addgene.org/101705

Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29132331

Proper citation: RRID:Addgene_101705 Copy   

•   Source: Addgene

http://www.addgene.org/101706

Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29132331

Proper citation: RRID:Addgene_101706 Copy   

•   Source: Addgene

http://www.addgene.org/101781

Species: Homo sapiens
Genetic Insert: CETN2 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_101781 Copy   

•   Source: Addgene

http://www.addgene.org/101782

Species: Homo sapiens
Genetic Insert: LAMP1 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_101782 Copy   

•   Source: Addgene

http://www.addgene.org/101821

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101821 Copy   

•   Source: Addgene

http://www.addgene.org/101784

Species: Homo sapiens
Genetic Insert: SLC25A17 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SLC25A17 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: RDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_101784 Copy   

•   Source: Addgene

http://www.addgene.org/101785

Species: Homo sapiens
Genetic Insert: TUBA1B Homology Arms with mTagRFPT-linker
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_101785 Copy   

•   Source: Addgene

http://www.addgene.org/101750

Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28886218

Proper citation: RRID:Addgene_101750 Copy   

•   Source: Addgene

http://www.addgene.org/101804

Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933

Proper citation: RRID:Addgene_101804 Copy   

•   Source: Addgene

http://www.addgene.org/101765

Vector Backbone Description: Vector Backbone:pMH-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877

Proper citation: RRID:Addgene_101765 Copy   

•   Source: Addgene

http://www.addgene.org/101768

Vector Backbone Description: Vector Backbone:pMH-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877

Proper citation: RRID:Addgene_101768 Copy   

•   Source: Addgene