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Species: Homo sapiens
Genetic Insert: Human Sacsin 1-339
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29945973
Proper citation: RRID:Addgene_75298 Copy
Species: Synthetic
Genetic Insert: Sacsin Ceratotherium simum simum 89-1366
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75331 Copy
Species: Mus musculus
Genetic Insert: eGFP-2A-mKv1.2
Vector Backbone Description: Backbone Marker:Bryan Roth; Backbone Size:4554; Vector Backbone:Addgene Plasmid #50465; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75295 Copy
Species: Homo sapiens
Genetic Insert: SLC24A5
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75214 Copy
Species: Synthetic
Genetic Insert: Sacsin Eptesicus fuscus 89-1368
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75335 Copy
Species: Mus musculus
Genetic Insert: SLC24A5
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75213 Copy
Species: Synthetic
Genetic Insert: Sacsin Leptonychotes weddellii 89-1367
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75334 Copy
Species: Homo sapiens
Genetic Insert: SLC24A4
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12379639
Comments: FLAG tag is inserted internal to the protein coding region, as annotated in the annotated sequence file.
Proper citation: RRID:Addgene_75212 Copy
Species: Synthetic
Genetic Insert: Sacsin Mustela putorius furo 94-1381
Vector Backbone Description: Backbone Marker:GE Healthcare ; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75333 Copy
Species: Homo sapiens
Genetic Insert: SLC24A4
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12379639
Proper citation: RRID:Addgene_75211 Copy
Species: Xenopus laevis
Genetic Insert: H2BK120C
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4640; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26912860
Proper citation: RRID:Addgene_75332 Copy
Species: Other
Genetic Insert: TRE-DEST
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27133796
Proper citation: RRID:Addgene_75339 Copy
Species: Homo sapiens
Genetic Insert: SLC8B1
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14625281
Proper citation: RRID:Addgene_75216 Copy
Species: Other
Genetic Insert: TRE-DEST
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27133796
Proper citation: RRID:Addgene_75336 Copy
Species: Homo sapiens
Genetic Insert: shRNA against hBcl10
Vector Backbone Description: Vector Backbone:pQCXIX (modified); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Produced 70-80 % KD (Western Blot) in Jurkat cells after RV-transduction and sort for GFPpositive cells.
Proper citation: RRID:Addgene_75248 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhance specificity (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75236 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc2
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75233 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75238
Proper citation: RRID:Addgene_75239 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
MUST BE COTRANSFECTED WITH PLASMID #75239
Proper citation: RRID:Addgene_75238 Copy
Species: Homo sapiens
Genetic Insert: sgRNA against NFATc1
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48138); Backbone Size:9703; Vector Backbone:PX458-pSpCas9(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27637333
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhance specificity (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).)
targets all isoforms.
Proper citation: RRID:Addgene_75237 Copy
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