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Species: Homo sapiens
Genetic Insert: HNF4A 3'UTR fragment
Vector Backbone Description: Backbone Size:6380; Vector Backbone:pRL-Con; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22140441
Comments: To measure regulatory potential of 3'UTR
Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.
Proper citation: RRID:Addgene_31452 Copy
Species: Homo sapiens
Genetic Insert: HNF4A 3'UTR fragment
Vector Backbone Description: Backbone Size:6380; Vector Backbone:pRL-Con; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22140441
Comments: To measure regulatory potential of 3'UTR
Proper citation: RRID:Addgene_31453 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Marker:Sikorski R., Hieter P.; Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31456 Copy
Vector Backbone Description: Backbone Size:6191; Vector Backbone:pyrFEKO; Vector Types:Floxed targeting vector for Trypanosome; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_31455 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Size:4970; Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31458 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31457 Copy
Species: Homo sapiens
Genetic Insert: phytanoyl-CoA hydroxylase interacting protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31578 Copy
Genetic Insert: linker
Vector Backbone Description: Backbone Size:4970; Vector Backbone:pRS413; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21722715
Comments: pRS plasmid series originally constructed by Sikorski and Hieter (Sikorski, R.S. and Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122 (1989), pp. 19-27.)
Proper citation: RRID:Addgene_31459 Copy
Species: Homo sapiens
Genetic Insert: DiGeorge syndrome critical region gene 6
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31564 Copy
Species: Danio rerio
Genetic Insert: pCS4+-P2A
Vector Backbone Description: Backbone Size:4146; Vector Backbone:pCS4; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21602908
Proper citation: RRID:Addgene_31566 Copy
Species: Homo sapiens
Genetic Insert: transgelin 2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31569 Copy
Species: Homo sapiens
Genetic Insert: tumor protein, translationally-controlled 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.
Proper citation: RRID:Addgene_31558 Copy
Species: Polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31494 Copy
Species: polyomavirus
Genetic Insert: Half genome of WU polyomavirus
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3957; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17480120
Proper citation: RRID:Addgene_31493 Copy
Species: Homo sapiens
Genetic Insert: RB
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20870719
Proper citation: RRID:Addgene_31496 Copy
Species: Homo sapiens
Genetic Insert: RB
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20870719
Proper citation: RRID:Addgene_31498 Copy
Genetic Insert: lactose repressor protein
Vector Backbone Description: Backbone Size:4540; Vector Backbone:pEMBL9'; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11266612
Comments: Parent plasmid was pEMBL9' with insert of extended LacI sequence from pIQ (Hare & Sadler, Gene 3 (1978) 269-278.
For expression: E. coli BLIM cells (or any E. coli WITHOUT lactose repressor in the genome or accompanying plasmid)
Proper citation: RRID:Addgene_31490 Copy
Species: E. coli variant
Genetic Insert: Lactose repressor with 11 amino acid C-terminal deletion
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLS1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is utilized to produce dimeric lactose repressor protein.
For Expression: E. coli BLIM cells or any E. coli WITHOUT LacI in the genome or another plasmid
Proper citation: RRID:Addgene_31492 Copy
Species: T7 phage
Genetic Insert: RNA polymerase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10610690
Comments: For expression: E. coli CV2
N823D mutation should not affect plasmid function.
Proper citation: RRID:Addgene_31491 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9364701
Comments: *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
Proper citation: RRID:Addgene_31598 Copy
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