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Species: Homo sapiens
Genetic Insert: CENPB poly(A) site
Vector Backbone Description: Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Proper citation: RRID:Addgene_120179 Copy
Species: Homo sapiens
Genetic Insert: CENPB CRISPRi guide RNA
Vector Backbone Description: Vector Backbone:pU6-sgGAL4-1; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Proper citation: RRID:Addgene_120214 Copy
Species: Homo sapiens
Genetic Insert: ILVBL poly(A) site
Vector Backbone Description: Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Comments: There is a single mutation in EGFP-G24D. Depositor confirms this does not affect plasmid function.
Proper citation: RRID:Addgene_120206 Copy
Species: Homo sapiens
Genetic Insert: N23 3'UTR
Vector Backbone Description: Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: PIK3CG 3'UTR in renilla luciferase reporter plasmid.
Proper citation: RRID:Addgene_12019 Copy
Species: Homo sapiens
Genetic Insert: ILVBL poly(A) site
Vector Backbone Description: Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Proper citation: RRID:Addgene_120207 Copy
Species: Homo sapiens
Genetic Insert: N22 3'UTR
Vector Backbone Description: Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: MDFIC 3'UTR in renilla luciferase reporter plasmid.
Proper citation: RRID:Addgene_12018 Copy
Species: Homo sapiens
Genetic Insert: TFE3 poly(A) site
Vector Backbone Description: Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Proper citation: RRID:Addgene_120202 Copy
Species: Homo sapiens
Genetic Insert: TFE3 poly(A) site
Vector Backbone Description: Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28525757
Proper citation: RRID:Addgene_120204 Copy
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31015513
Proper citation: RRID:Addgene_120274 Copy
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:; Bacterial Resistance:Ampicillin + 1% Glucose
Defining Citation: PMID:31015513
Proper citation: RRID:Addgene_120276 Copy
Species: Mus musculus
Genetic Insert: Lipin-1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30718857
Proper citation: RRID:Addgene_120277 Copy
Species: Mus musculus
Genetic Insert: Lipin-1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30718857
Proper citation: RRID:Addgene_120278 Copy
Genetic Insert: sfGFP/mScarletP2A-mcs
Vector Backbone Description: Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that sfGFP can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A
The associated publication utilized mNeon instead of sfGFP/mClover3, but mNeon has is exceedingly difficult to share. Therefore, these two plasmids (120261 and 120262) are made available, using sfGFP and mClover3 instead of mNeon. These plasmids have been tested, and are equivalent to the mNeon variant.
Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_120261 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: DYSCKO cassette
Vector Backbone Description: Backbone Size:5500; Vector Backbone:p426; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30097473
Comments: See Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast. (PMID: 30097473) for detailed protocols for gRNA cloning and mutagenesis protocol.
Proper citation: RRID:Addgene_120263 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pDYSCKO cassette-ADE1 targeting guide
Vector Backbone Description: Backbone Size:6467; Vector Backbone:pDYSCKO; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30097473
Comments: See Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast. (PMID: 30097473) for detailed protocols for gRNA cloning and mutagenesis protocol.
Proper citation: RRID:Addgene_120264 Copy
Species: Aequorea Victoria
Genetic Insert: EmGFP
Vector Backbone Description: Backbone Size:2500; Vector Backbone:pEMBL8; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30582449
Proper citation: RRID:Addgene_120267 Copy
Genetic Insert: EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27292633
Proper citation: RRID:Addgene_120268 Copy
Genetic Insert: VD_CRISPR
Vector Backbone Description: Backbone Size:2517; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30709710
Proper citation: RRID:Addgene_120370 Copy
Genetic Insert: Akaluc (human optimized)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3928; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34650390
Comments: The akaluc gene was synthesized with human/murine codon optimization from the protein amino acid sequenced provided by Iwano et. al. Science 2018 as a more sensitive luciferase enzyme for live animal imaging in vivo with the substrate Akalumine-HCl. After synthesis, the gene was cloned and cloned into the eGFP-N1 (Addgene 6085-1) using restriction enzyme cloning.
Proper citation: RRID:Addgene_120371 Copy
Species: Human Cytomegalovirus (HHV-5)
Genetic Insert: UL91
Vector Backbone Description: Backbone Size:6168; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30305361
Proper citation: RRID:Addgene_120375 Copy
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