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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376751
Comments: This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4014-4047
Proper citation: RRID:Addgene_48976 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5050; Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Bleocin (Zeocin)
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49022 Copy
Species: Homo sapiens
Genetic Insert: MALT1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22309193
Proper citation: RRID:Addgene_48971 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7634; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 3633-4493.
Proper citation: RRID:Addgene_49026 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8347; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 6364-7224.
Proper citation: RRID:Addgene_49025 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7638; Vector Backbone:pYES2/CT; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
The ampicillin resistance cassette is located at bp# 3637-4497.
Proper citation: RRID:Addgene_49024 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48964 Copy
Species: Danio rerio
Genetic Insert: friend leukemia integration 1b
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2515; Vector Backbone:pdonr221; Vector Types:gateway middle entry; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24036310
Proper citation: RRID:Addgene_49019 Copy
Species: Homo sapiens
Genetic Insert: SUMO3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48963 Copy
Species: E. coli
Genetic Insert: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA
This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).
Proper citation: RRID:Addgene_49018 Copy
Vector Backbone Description: Backbone Marker:life technologies; Vector Backbone:pMA-T; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Proper citation: RRID:Addgene_48961 Copy
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Proper citation: RRID:Addgene_49016 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Comments: Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
Proper citation: RRID:Addgene_48966 Copy
Species: Homo sapiens
Genetic Insert: SUMO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21247896
Proper citation: RRID:Addgene_48965 Copy
Genetic Insert: cas9
Vector Backbone Description: Backbone Marker:life technologies; Backbone Size:4959; Vector Backbone:pDEST; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013562
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_48960 Copy
Species: Homo sapiens
Genetic Insert: dCas9_VP64 (human-codon-optimized)
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4000; Vector Backbone:phi-Yellow-Dest; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23977949
Comments: L591M mutation with dCas9
Proper citation: RRID:Addgene_49015 Copy
Vector Backbone Description: Backbone Marker:Dankort lab, unpublished; Backbone Size:7396; Vector Backbone:gQxiPuro; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48958 Copy
Genetic Insert: Multiple Cloning Site
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONRP2RP3; Vector Types:gateway 3' Entry; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24036310
Comments: There is a single base mismatch between Addgene's quality control sequence and the full plasmid reference sequence. The change is in backbone region and should not affect function.
Proper citation: RRID:Addgene_49004 Copy
Vector Backbone Description: Backbone Marker:Messing Lab, PMID 1905257; Backbone Size:3772; Vector Backbone:pOK1/2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48942 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA',fadR; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
fadE- fadR->CAT, plTet0-> (tesA and fadD), fadR overexpression
Proper citation: RRID:Addgene_48939 Copy
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