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Species: Synthetic
Genetic Insert: xCas9 3.7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108379 Copy
Species: Homo sapiens
Genetic Insert: PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108376 Copy
Species: Bos taurus
Genetic Insert: p110 KD
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR. The kinase dead mutant has an additional BamHI site at D915S.
Proper citation: RRID:Addgene_10837 Copy
Species: Bos taurus
Genetic Insert: p110
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR.
Proper citation: RRID:Addgene_10836 Copy
Species: Homo sapiens
Genetic Insert: Arf1 Q71L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11773070
Proper citation: RRID:Addgene_10832 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3457; Vector Backbone:pBluescript; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27913727
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
Proper citation: RRID:Addgene_108352 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_108342 Copy
Species: Synthetic
Genetic Insert: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Comments: Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
Proper citation: RRID:Addgene_108356 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Species: Synthetic
Genetic Insert: RspCas13d
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5390; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29551514
Comments: For commercial use, please contact Arbor Biotechnologies at [email protected].
Proper citation: RRID:Addgene_108305 Copy
Species: A. victoria
Genetic Insert: GFP under control of arabinose-inducible promoter pBAD
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3541; Vector Backbone:pJ401; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108315 Copy
Species: mycobacterial phage
Genetic Insert: Che9C RecT
Vector Backbone Description: Backbone Marker:Dirk Schnappinger and Sabine Ehrt; Vector Backbone:pUV15tet-O; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30538179
Comments: The plasmid is induced with 500 ng/ml anhydrotetracycline to induce expression of RecT and Bxb1 Integrase for performing the ORTBIT protocol.
Proper citation: RRID:Addgene_108319 Copy
Species: Homo sapiens
Genetic Insert: Shugoshin 1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25892238
Proper citation: RRID:Addgene_108494 Copy
Species: Homo sapiens
Genetic Insert: AuroraB
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22267324
Comments: Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.
Proper citation: RRID:Addgene_108493 Copy
Species: Homo sapiens
Genetic Insert: AuroraB
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22267324
Comments: Addgene's sequencing results found M298T in AuroraB compared to the NCBI reference. The depositing lab states that there is a SNP at this location, and the discrepancy should not affect plasmid function.
Proper citation: RRID:Addgene_108488 Copy
Species: Homo sapiens
Genetic Insert: INCENP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19150808
Proper citation: RRID:Addgene_108487 Copy
Species: Homo sapiens
Genetic Insert: SOST
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908424
Proper citation: RRID:Addgene_10842 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pCAG-WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Comments: Addgene QC NGS identified some sequence discrepancies in the plasmid backbone, relative to the depositing lab's provided sequence. Please see the Sequences webpage for additional information.
Proper citation: RRID:Addgene_108423 Copy
Species: Synthetic
Genetic Insert: Archon1-KGC-EGFP-ER2
Vector Backbone Description: Vector Backbone:pAAV-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108422 Copy
Species: Human papillomavirus
Genetic Insert: HPV11 E6
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1661671
Comments: See author's map.
Proper citation: RRID:Addgene_10848 Copy
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