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Genetic Insert: ECFP(loxP)MCS
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Comments: Cre reporter: from blue protein fluorescence to gene of interested inserted into multiple cloning site (MCS)
Proper citation: RRID:Addgene_31376 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Proper citation: RRID:Addgene_31411 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Proper citation: RRID:Addgene_31414 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Comments: *M93I and R185K mutations compared to NP_542409.1
Proper citation: RRID:Addgene_31416 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Proper citation: RRID:Addgene_31418 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Proper citation: RRID:Addgene_31417 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:8100; Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17873885
Comments: *S171T mutation when compared to NP_003335.1
Proper citation: RRID:Addgene_31419 Copy
Genetic Insert: Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31483 Copy
Genetic Insert: Traffic Light Reporter 1.1 (Sce Target) EF Puro
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31482 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans glutamate-gated chloride channel alpha, crystallized construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pFastbac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21572436
Proper citation: RRID:Addgene_31488 Copy
Genetic Insert: Delta-selective C kinase activity reporter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20959447
Proper citation: RRID:Addgene_31526 Copy
Species: Homo sapiens
Genetic Insert: YFP-HA-Protein Kinase D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19581308
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.
Proper citation: RRID:Addgene_31527 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2760; Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31472 Copy
Species: Homo sapiens
Genetic Insert: small fragment nuclease
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pQTEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence.
The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
Proper citation: RRID:Addgene_31593 Copy
Species: fluorescent proteins
Genetic Insert: eYFP and mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:2850; Vector Backbone:pTRETightBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857679
Comments: mCherry fused 3'UTR composed of SLC6A1, which contains three binding sites for the neuronal miRNA miR-218
Proper citation: RRID:Addgene_31471 Copy
Species: Homo sapiens
Genetic Insert: preimplantation protein 3
Vector Backbone Description: Backbone Size:3460; Vector Backbone:pQStrep2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15998469
Comments: Please note that there is a small gap between Addgene's quality control sequence and the reference sequence provided by the depositor. The gap is downstream of the ORF and should not affect function.
Proper citation: RRID:Addgene_31592 Copy
Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Marker:British Biotechnology; Backbone Size:5300; Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21631110
Proper citation: RRID:Addgene_31473 Copy
Genetic Insert: SFFV d14 GFP donor EF1s I-SceI
Vector Backbone Description: Backbone Size:5092; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31476 Copy
Genetic Insert: SFFV d14GFP
Vector Backbone Description: Backbone Size:5516; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31475 Copy
Genetic Insert: SFFV HA NLS Y2 K227M T2A BFP
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCVL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_31478 Copy
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