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Species: Synthetic
Genetic Insert: D-Dummy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
D-dummy = 2252-2297bp
BsaI site #2 = 2298-2308bp
Proper citation: RRID:Addgene_48834 Copy
Species: Synthetic
Genetic Insert: Linker:GFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
linker-GFP = 2252-3072bp
BsaI site #2 = 3073-3083bp
Proper citation: RRID:Addgene_48833 Copy
Species: Arabidopsis thaliana
Genetic Insert: WUS
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
WUS coding sequence = 2252-3129bp
BsaI site #2 = 3130-3140bp
Proper citation: RRID:Addgene_48824 Copy
Species: Synthetic
Genetic Insert: signal sequence ER
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
ER signal sequence = 2252-2320bp
BsaI site #2 = 2321-2331bp
Proper citation: RRID:Addgene_48823 Copy
Species: Synthetic
Genetic Insert: SV40 nuclear localization signal
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
SV40 nuclear localization signal = 2252-2275bp
BsaI site #2 = 2276-2286bp
Proper citation: RRID:Addgene_48822 Copy
Species: Synthetic
Genetic Insert: mCherry:Linker
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
mCherry-linker = 2252-3060bp
BsaI site #2 = 3061-3071bp
Proper citation: RRID:Addgene_48819 Copy
Genetic Insert: sodA LuxR
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:22178928
Proper citation: RRID:Addgene_48890 Copy
Genetic Insert: sfGFP and luxI
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22178928
Comments: * The presence of glucose will reduce the amount of expression from the Lux promoter driving GFP and other genes. Omitting the glucose could potentially make the cell overburdened.
Proper citation: RRID:Addgene_48887 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48924 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48923 Copy
Genetic Insert: ndhII and LuxR
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:22178928
Proper citation: RRID:Addgene_48889 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48922 Copy
Genetic Insert: aiiA
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22178928
Proper citation: RRID:Addgene_48888 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48921 Copy
Genetic Insert: lacI with ssrA tag
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18971928
Proper citation: RRID:Addgene_48882 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:6992; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49050 Copy
Genetic Insert: Sh ble
Vector Backbone Description: Backbone Marker:Life Technologies (Invitrogen); Backbone Size:2500; Vector Backbone:pDONR221; Vector Types:Gateway attB1 & attB2; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19432966
Comments: Please note that Addgene's sequencing results found a few differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_48995 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:6986; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49049 Copy
Species: E. coli
Genetic Insert: Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed)
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA
This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.
A mutS+ version of this strain will be deposited with addgene soon.
Genome sequence deposited at NCBI (accession # CP006698)
Proper citation: RRID:Addgene_48998 Copy
Vector Backbone Description: Backbone Marker:Joung Lab; Backbone Size:8550; Vector Backbone:unknown; Vector Types:Mammalian Expression, TALE LSD1 Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24013198
Proper citation: RRID:Addgene_49044 Copy
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