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pMaCTag-Z16
 
Resource Report
Resource Website 		RRID:Addgene_120059 Dronpa3 Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pAG189 CASC2 3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_12017 N21 3'UTR Homo sapiens Ampicillin PMID:16308420 CASC2 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:17 0
pAG186 NID1 3'UTR
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_12016 N19 3'UTR Homo sapiens Ampicillin PMID:16308420 NID1 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:17 1
pAG185 PDLIM5 3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_12015 N18 3'UTR Homo sapiens Ampicillin PMID:16308420 PDLIM5 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pAG184 NEK6 3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_12014 N17 3'UTR Homo sapiens Ampicillin PMID:16308420 NEK6 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pAG199 QKI 3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_12011 QKI 3'UTR Homo sapiens Ampicillin PMID:16308420 QKI 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pAG198 PRKD1 3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_12010 PRKD1 3'UTR Homo sapiens Ampicillin PMID:16308420 PRKD1 3'UTR in renilla luciferase reporter plasmid. Backbone Marker:pIS2 derived from pRL-SV40 (Promega); Backbone Size:3700; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
eGFP-proα2(I)-L
 
Resource Report
Resource Website 		RRID:Addgene_120160 Type I procollagen α2 chain Mus musculus Ampicillin PMID:30287488 These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Col1a2 exons 2-3 replaced by fluorescent tag Addgene 2026-10-24 02:04:17 0
pMaCTag-P29
 
Resource Report
Resource Website 		RRID:Addgene_120040 Strep-II Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pMaCTag-P30
 
Resource Report
Resource Website 		RRID:Addgene_120041 VSV-G Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pCMV-GFP-CENPBpA
 
Resource Report
Resource Website 		RRID:Addgene_120162 CENPB poly(A) site Homo sapiens Ampicillin PMID:28525757 Vector Backbone:pCMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:17 0
mCh-KIF5A*-strep
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_120163 kinesin family member 5A Mus musculus Kanamycin PMID:31100061 Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Truncated KIF5A: 1-559 aa Addgene 2026-10-24 02:04:17 1
pMaCTag-P32
 
Resource Report
Resource Website 		RRID:Addgene_120043 S-Tag Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
mCh-KIF1A*-strep
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_120165 kinesin family member 1A Mus musculus Kanamycin PMID:31100061 Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Truncated KIF1A: 1-405 aa Addgene 2026-10-24 02:04:17 1
pMaCTag-Z02
 
Resource Report
Resource Website 		RRID:Addgene_120045 TagBFP Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
mCh-BICD2*-strep
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_120168 BICD cargo adaptor 2 Mus musculus Kanamycin PMID:31100061 Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Truncated BICD2: 15-595 aa Addgene 2026-10-24 02:04:17 1
pMaCTag-Z05
 
Resource Report
Resource Website 		RRID:Addgene_120048 eGFP Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pMaCTag-P34
 
Resource Report
Resource Website 		RRID:Addgene_120030 3xFLAG Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pMaCTag-P22
 
Resource Report
Resource Website 		RRID:Addgene_120033 SNAP Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 0
pMaCTag-P26
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_120037 mAID-Clover Homo sapiens Ampicillin PMID:32406907 Template plasmid for C-terminal PCR tagging of mammalian genes. For M1 and M2 tagging oligo design please visit www.pcr-tagging.com Please visit https://www.biorxiv.org/content/early/2018/11/20/473876 for BioRxiv preprint Backbone Size:2412; Vector Backbone:pFA6a; Vector Types:PCR Template; Bacterial Resistance:Ampicillin Addgene 2026-10-24 02:04:16 1

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