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Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:6767; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: The pFBOH-MHL vector is a derivative of the pFBOH-LIC vector (SGC). It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host. This vector adds an 18 amino acid N-terminal fusion tag containing a 6X His followed by a TEV cleavage site. Two stop codons are included in the vector at the C-terminal cloning site.
Insertion of a DNA sequence into the cloning/expression region is performed using Clontech’s In-fusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of a target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose.
Proper citation: RRID:Addgene_62304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: OSM1-EGFP fusion
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62387 Copy
Species: Other
Genetic Insert: mCherry-BirA
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62388 Copy
Species: Synthetic
Genetic Insert: su9-tagBFP
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62383 Copy
Species: Synthetic
Genetic Insert: tev 3x FLAG
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCRII blunt topo; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62384 Copy
Genetic Insert: sgRNA + 2x PP7 RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62317 Copy
Genetic Insert: sgRNA + 1x com RNA binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62318 Copy
Genetic Insert: sgRNA + 1x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62313 Copy
Genetic Insert: sgRNA + 2x MS2 binding module
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62314 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-HindIII-PstI-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62397 Copy
Species: Synthetic
Genetic Insert: MluI-XhoI-PvuII-BglII-SalI MCS
Vector Backbone Description: Backbone Marker:Neveu lab, Addgene plasmid #62394; Backbone Size:1945; Vector Backbone:MXS_chaining vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62398 Copy
Vector Backbone Description: Backbone Size:1945; Vector Backbone:Custom Backbone; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25909630
Proper citation: RRID:Addgene_62394 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62327 Copy
Genetic Insert: sgRNA + 1x MS2 binding module
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62328 Copy
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_62329 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62325 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5144; Vector Backbone:pDONR P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62289 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62338 Copy
Genetic Insert: sgRNA + 2x PP7
Vector Backbone Description: Vector Backbone:MP177_U6 (derived from pSico); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62334 Copy
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