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Vector Backbone Description: Backbone Size:2051; Vector Backbone:pENTR1A; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme. It serves to create intermediate supermodules with plant resistance cassettes.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 358-368bp
BsaI site #2 = 1796-1806bp
Proper citation: RRID:Addgene_48865 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48862 Copy
Vector Backbone Description: Backbone Size:2492; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 602-612bp
BsaI site #2 = 2040-2050bp
Proper citation: RRID:Addgene_48867 Copy
Genetic Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Vector Backbone Description: Vector Backbone:pZE41 backbone (specR), fadD, tesA'; Vector Types:; Bacterial Resistance:Spectinomycin
Comments: strain contains pZE41 backbone (specR), fadD, tesA'
galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
Proper citation: RRID:Addgene_48900 Copy
Vector Backbone Description: Backbone Size:2492; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Gentamicin
Defining Citation: PMID:24376629
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang.
Please note that a transposon insertion is present in the chloramphenicol resistance promoter. This insertion does not interfere with plasmid function. There is an additional BsaI site at bp# 1889-1894 which is irrelevant for GreenGate reactions with this plasmid.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 640-650bp
transposon integration = 747-2077bp
BsaI site #2 = 3414-3419bp
Proper citation: RRID:Addgene_48866 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48861 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48860 Copy
Species: Arabidopsis thaliana
Genetic Insert: At1g04880 terminator
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
At1g04480 terminator = 2252-2758bp
BsaI site #2 = 2759-2769bp
Proper citation: RRID:Addgene_48854 Copy
Species: Synthetic
Genetic Insert: Linker:BFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
linker-BFP = 2252-3066bp
BsaI site #2 = 3067-3077bp
Proper citation: RRID:Addgene_48853 Copy
Species: Synthetic
Genetic Insert: H-A adapter - short
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
H-A adapter = 2252-2271bp
BsaI site #2 = 2272-2282bp
Proper citation: RRID:Addgene_48851 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48856 Copy
Species: Synthetic
Genetic Insert: F-H adapter - short
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
F-H adapter = 2252-2271bp
BsaI site #2 = 2272-2282bp
Proper citation: RRID:Addgene_48850 Copy
Species: Synthetic
Genetic Insert: pMAS::SulfadiazineR::t35S
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
Sulfadiazine resistance cassette with MAS promoter and 35S terminator = 2252-3882bp
BsaI site #2 = 3883-3893bp
Proper citation: RRID:Addgene_48849 Copy
Species: Synthetic
Genetic Insert: pNOS::BastaR::tNOS
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BASTA resistance cassette with NOS promoter and terminator = 2252-3334bp
BsaI site #2 = 3335-3345bp
Proper citation: RRID:Addgene_48848 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ10 terminator
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
UBQ10 terminator = 2252-2879bp
BsaI site #2 = 2880-2890bp
Proper citation: RRID:Addgene_48841 Copy
Species: Arabidopsis thaliana
Genetic Insert: linker:nuclear localization signal
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
linker-nuclear localization signal = 2252-2550bp
BsaI site #2 = 2551-2561bp
Proper citation: RRID:Addgene_48837 Copy
Species: Synthetic
Genetic Insert: 3xmCherry
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
3x mCherry coding sequence = 2252-4491bp
BsaI site #2 = 4492-4502bp
Proper citation: RRID:Addgene_48831 Copy
Species: Synthetic
Genetic Insert: 3xGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
3x GFP coding sequence = 2252-4518bp
BsaI site #2 = 4519-4529bp
Proper citation: RRID:Addgene_48830 Copy
Species: Synthetic
Genetic Insert: SV40 NLS
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
SV40 nuclear localization signal = 2252-2277bp
BsaI site #2 = 2278-2288bp
Proper citation: RRID:Addgene_48836 Copy
Species: Synthetic
Genetic Insert: Linker-mCherry
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed for Golden Gate cloning using the BsaI enzyme.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
linker-mCherry = 2252-3063bp
BsaI site #2 = 3064-3074bp
Proper citation: RRID:Addgene_48835 Copy
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