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Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62203 Copy
Genetic Insert: PCP-VP64
Vector Backbone Description: Vector Backbone:pNH603; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62280 Copy
Genetic Insert: COM-VP64
Vector Backbone Description: Vector Backbone:pNH603; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62281 Copy
Species: Synthetic
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62166 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62200 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62168 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62201 Copy
Genetic Insert: MCP-VP64
Vector Backbone Description: Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25533786
Proper citation: RRID:Addgene_62282 Copy
Species: Synthetic
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62162 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62284 Copy
Species: Synthetic
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62164 Copy
Species: Synthetic
Genetic Insert: scramble sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62285 Copy
Species: Mus musculus
Genetic Insert: Nxph4-2A-CreERT2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2850; Vector Backbone:pBluescriptII SK+; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25071457
Proper citation: RRID:Addgene_62219 Copy
Species: Synthetic
Genetic Insert: epNTR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24848468
Proper citation: RRID:Addgene_62213 Copy
Vector Backbone Description: Backbone Size:2644; Vector Backbone:puc19; Vector Types:Zebrafish transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25628360
Comments: Zebrafish transgenesis vector: tol1 arms, QUAS-E1b promoter, ubiquitin C intron, multiple cloning site, ocean pout antifreeze protein terminator.
Co-inject with tol1 (not tol2) transposase.
Proper citation: RRID:Addgene_62216 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62170 Copy
Genetic Insert: Fok1-dCas9
Vector Backbone Description: Backbone Marker:gift from Silvia Aldaz; Backbone Size:12429; Vector Backbone:pact; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Please visit crisprflydesign.org for more information.
Please acknowledge Fillip Port and Simon Bullock when publishing work derived from the use of this plasmid.
Proper citation: RRID:Addgene_62211 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, MuLE Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62179 Copy
Species: Synthetic
Genetic Insert: CreERT2
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62174 Copy
Species: Synthetic
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2000; Vector Backbone:Trc99a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25636838
Proper citation: RRID:Addgene_62226 Copy
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