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Species: Lachnospiraceae bacterium
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Comments: The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431
Proper citation: RRID:Addgene_183074 Copy
Genetic Insert: FluoSTEP-JNKAR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5359; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34020947
Proper citation: RRID:Addgene_181857 Copy
Genetic Insert: cp-PKN-mRuby2-GFP(1-0)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5359; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34020947
Proper citation: RRID:Addgene_181859 Copy
Species: Homo sapiens
Genetic Insert: PEN2
Vector Backbone Description: Backbone Size:8300; Vector Backbone:pBoBi; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35197629
Proper citation: RRID:Addgene_183640 Copy
Species: Homo sapiens
Genetic Insert: PEN2
Vector Backbone Description: Backbone Size:8300; Vector Backbone:pBoBi; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35197629
Proper citation: RRID:Addgene_183641 Copy
Species: Homo sapiens
Genetic Insert: ATP6AP1
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35197629
Proper citation: RRID:Addgene_183643 Copy
Species: Cowpea severe mosaic virus
Genetic Insert: CPSMV RNA1 cDNA with 4 introns
Vector Backbone Description: Backbone Marker:Qu lab; Backbone Size:7869; Vector Backbone:pAIDE (a pCambia1300 drivative); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36307846
Comments: An upgraded version of this plasmid is available at Addgene. Please see Plasmid #254915 (https://www.addgene.org/254915/).
Proper citation: RRID:Addgene_184267 Copy
Species: Cowpea severe mosaic virus
Genetic Insert: CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
Vector Backbone Description: Backbone Marker:Qu lab; Backbone Size:7872; Vector Backbone:pAIDE (a pCambia1300 drivative); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36307846
Comments: An upgraded version of this plasmid is available at Addgene. Please see Plasmid #254916 (https://www.addgene.org/254916/).
Proper citation: RRID:Addgene_184268 Copy
Species: Homo sapiens
Genetic Insert: AKAP79-AKAR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5409; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33201801
Proper citation: RRID:Addgene_181957 Copy
Species: Rattus norvegicus
Genetic Insert: EGFP-AC8(1-106)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5430; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33201801
Proper citation: RRID:Addgene_181958 Copy
Species: Mus musculus
Genetic Insert: anti-Kv2.1 K+ channel pS586 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2895692. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182099 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183222 Copy
Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:Modified pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35659325
Comments: This Cas12c bacterial expression plasmid was originally created by Harrington et al., 2020 and now modified (R965H) to match with the wild-type protein sequence of Cas12c_4.
Proper citation: RRID:Addgene_183069 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159829 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA mosTI hygroR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159827 Copy
Species: Caenorhabditis elegans
Genetic Insert: array co-injection fragment mosTI hygroR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159828 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159825 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI hygroR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159826 Copy
Genetic Insert: Cas12 Guide (crAAVS1_6)
Vector Backbone Description: Vector Backbone:PB; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35637351
Proper citation: RRID:Addgene_183624 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI unc-119 target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159821 Copy
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