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Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62114 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62116 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = aspC tyrB
Precursor strain = RF3
modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe)
++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation).
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_62072 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62110 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = aspC tyrB trpA trpB
Precursor strain = RF4
modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, Trp, (Phe)
++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation).
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_62078 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23609977
Comments: Includes a thrombin cleavage site
Proper citation: RRID:Addgene_62079 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62113 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = proC
Precursor strain = BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Pro
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_62074 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = asnA asnB
Precursor strain = BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Asn
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_62075 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62125 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62128 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15824059
Comments: Includes a thrombin cleavage site
Proper citation: RRID:Addgene_62082 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15824059
Comments: Includes a thrombin cleavage site
Proper citation: RRID:Addgene_62083 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62088 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62089 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62123 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62124 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62084 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62086 Copy
Species: Synthetic
Genetic Insert: non-silencing (scrambled) shRNA
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Comments: Please note that the n.s. shRNA contains an additional "C" nucleotide compared to the depositors reference sequence. The sequence between AgeI and EcoRI is 5' -ccggtcCtaaggttaagtcgccctcgctcgagcgagggcgacttaaccttaggtttttg - 3'.
Proper citation: RRID:Addgene_62136 Copy
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