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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMuLE ENTR U6-miR-30 L1-L4 Resource Report Resource Website |
RRID:Addgene_62114 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 0 | ||||
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pMuLE ENTR U6-miR-30 L5-L4 Resource Report Resource Website |
RRID:Addgene_62116 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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RF4 Resource Report Resource Website |
RRID:Addgene_62072 | none | None | PMID:26577727 | Genotype = aspC tyrB Precursor strain = RF3 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:49 | 0 | ||
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pMuLE ENTR 7SK L1-L4 Resource Report Resource Website |
RRID:Addgene_62110 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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RF13 Resource Report Resource Website |
RRID:Addgene_62078 | none | None | PMID:26577727 | Genotype = aspC tyrB trpA trpB Precursor strain = RF4 modified from the parent Escherichia coli BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asp++++, Tyr, Trp, (Phe) ++++RF4, RF5, and RF13 strains have knockouts in both aspC and tyrB genes and therefore require the presence of L-Asp (but not L-Glu) for growth in M63 minimal medium. These strains can be used for selective labeling of selected aromatic amino acid(s) such as Tyr (RF4, RF5, RF13) and/or Trp (RF13). We have NOT tested if these strains could also be used for selective Asp labeling studies (under investigation). Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:50 | 0 | ||
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pET28-p53 (1-73) (K24N) Resource Report Resource Website |
RRID:Addgene_62079 | p53 | Homo sapiens | Kanamycin | PMID:23609977 | Includes a thrombin cleavage site | Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | contains TAD residues 1-73; K24N | 2026-08-15 01:17:49 | 0 |
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pMuLE ENTR U6-miR-30 L1-R5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62113 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 1 | ||||
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RF6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62074 | none | None | PMID:26577727 | Genotype = proC Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Pro Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:49 | 1 | ||
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RF8 Resource Report Resource Website |
RRID:Addgene_62075 | none | None | PMID:26577727 | Genotype = asnA asnB Precursor strain = BL21 CodonPlus (DE3)-RIL Selective amino acid labeling (and/or requirement) = Asn Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:50 | 0 | ||
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pMuLE ENTR CMV/TO-miR-30 R4-R3 Resource Report Resource Website |
RRID:Addgene_62125 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 0 | ||||
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pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62128 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 1 | ||||
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pET28-p53 (1-73) (72R) Resource Report Resource Website 1+ mentions |
RRID:Addgene_62082 | p53 | Homo sapiens | Kanamycin | PMID:15824059 | Includes a thrombin cleavage site | Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | contains TAD residues 1-73; 72R | 2026-08-15 01:17:49 | 1 |
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pET28-p53 (1-73) (72P) Resource Report Resource Website |
RRID:Addgene_62083 | p53 | Homo sapiens | Kanamycin | PMID:15824059 | Includes a thrombin cleavage site | Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET28A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | contains TAD residues 1-73; 72P | 2026-08-15 01:17:49 | 0 |
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pMuLE ENTR MCS L3-L2 Resource Report Resource Website |
RRID:Addgene_62088 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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pMuLE ENTR MCS L5-L4 Resource Report Resource Website |
RRID:Addgene_62089 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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pMuLE ENTR CMV/TO-miR-30 L5-L2 Resource Report Resource Website |
RRID:Addgene_62123 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 0 | ||||
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pMuLE ENTR CMV/TO-miR-30 L1-L4 Resource Report Resource Website |
RRID:Addgene_62124 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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pMuLE ENTR MCS L1-R5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_62084 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 3 | ||||
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pMuLE ENTR MCS R4-R3 Resource Report Resource Website |
RRID:Addgene_62086 | Kanamycin | PMID:25751063 | Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:49 | 0 | ||||
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pMuLE ENTR U6 n.s shRNA L1-L4 Resource Report Resource Website |
RRID:Addgene_62136 | non-silencing (scrambled) shRNA | Synthetic | Kanamycin | PMID:25751063 | Please note that the n.s. shRNA contains an additional "C" nucleotide compared to the depositors reference sequence. The sequence between AgeI and EcoRI is 5' -ccggtcCtaaggttaagtcgccctcgctcgagcgagggcgacttaaccttaggtttttg - 3'. | Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:50 | 0 |
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