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Species: Pseudomonas putida F1
Genetic Insert: CymR'
Vector Backbone Description: Backbone Marker:Biedendieck et al., 2007; Vector Backbone:pRBBm34; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30392122
Proper citation: RRID:Addgene_119871 Copy
Genetic Insert: pConstitutive_Che9c60 and Che9c61
Vector Backbone Description: Vector Backbone:pB264/pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119879 Copy
Genetic Insert: pBAD_dCas9sth1
Vector Backbone Description: Vector Backbone:pAL5000/pMB1 shuttle vector; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119880 Copy
Species: Homo sapiens
Genetic Insert: Signal transducer and activator of transcription 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6000; Vector Backbone:pcDNA3-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note hSTAT1 in this plasmid contains the mutations A188T and Q718R compared to GenBank BC002704. The impact of these mutations on plasmid functionality is unknown.
Proper citation: RRID:Addgene_11987 Copy
Species: Homo sapiens
Genetic Insert: MMP7
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6000; Vector Backbone:pcDNA3-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11989 Copy
Vector Backbone Description: Backbone Size:7298; Vector Backbone:pCIG3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31594807
Comments: Please note that though there are some discrepancies between the backbone sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as described.
Proper citation: RRID:Addgene_119863 Copy
Species: Escherichia coli str. K-12
Genetic Insert: outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
Vector Backbone Description: Vector Backbone:pET503; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30275279
Proper citation: RRID:Addgene_119860 Copy
Species: Synthetic
Genetic Insert: CymRV5-nls-GFP
Vector Backbone Description: Backbone Size:5152; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119906 Copy
Species: Synthetic
Genetic Insert: cuOx144 array
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pDEST-Bxb1attB-loxP-PGK-puro-loxP; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please note there are a few ambiguous bases in Addgene's NGS result. This is due to NGS having difficulty assembling repetitive sequences.
The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation. Two separate sequencing results shown above illustrate variable number of repeats.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119903 Copy
Species: Danio rerio
Genetic Insert: myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3121; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358
Proper citation: RRID:Addgene_119867 Copy
Species: Mus musculus
Genetic Insert: ret
Vector Backbone Description: Vector Backbone:pBS; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20064391
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse C-ret. Linearize the plasmid with NotI and use T7 to transcribe the ~1kb probe.
Proper citation: RRID:Addgene_119900 Copy
Species: Danio rerio
Genetic Insert: NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3008; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358
Proper citation: RRID:Addgene_119868 Copy
Species: Mycobacteriophage Bxb1
Genetic Insert: Mammalian codon optimized Bxb1 integrase
Vector Backbone Description: Backbone Size:4905; Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119901 Copy
Species: Synthetic
Genetic Insert: HA-tdMS2cp-tagRFP-Tx2-nls
Vector Backbone Description: Backbone Size:3526; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119908 Copy
Species: Synthetic
Genetic Insert: TetRFlag-nls-tdTom
Vector Backbone Description: Backbone Size:5670; Vector Backbone:ePiggyBac-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119909 Copy
Genetic Insert: dCas13b-GGS-NYTHDF1
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280
Proper citation: RRID:Addgene_119855 Copy
Species: Escherichia coli (strain K12)
Genetic Insert: precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
Vector Backbone Description: Vector Backbone:pBR322 with T7 promoter; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30275279
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_119853 Copy
Genetic Insert: dCas13b-GGS-Sun
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280
Proper citation: RRID:Addgene_119858 Copy
Genetic Insert: dCas13b-GGS-NYTHDF2(100-200)
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280
Proper citation: RRID:Addgene_119856 Copy
Genetic Insert: dCas13b-GGS-NYTHDF1(100-200)
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280
Proper citation: RRID:Addgene_119857 Copy
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