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Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182656 Copy
Species: Homo sapiens
Genetic Insert: Calnexin
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37322101
Proper citation: RRID:Addgene_183987 Copy
Species: Mus musculus
Genetic Insert: wild type mouse neurofilament light chain
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182659 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179895 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K468TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182658 Copy
Species: Methanosarcina mazei
Genetic Insert: codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Comments: Addgene QC NGS analysis identified 3X U6 tRNA M15, rather than 4X. The depositor has confirmed that this should have no influence on the functionality of the plasmid.
Proper citation: RRID:Addgene_182652 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mCherry Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179898 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon mNeonGreen Rab11b knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179899 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179893 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for mNeonGreen knock in to Rab11a.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179890 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5236; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35639414
Comments: Protein sequence is MGSS + HHHHHH + GGG + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1).
Confers kanamycin-resistance.
Backbone vector is pET-28a.
Construct design by Ryan Boyd.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179290 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5913; Vector Backbone:pET His6 MBP TEV LIC (2M-T) Addgene # 2970; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639414
Comments: Protein sequence is MKSS + HHHHHH + GSS + maltose binding protein (aa 27-393 of AAB59056) + NGIE + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + N + human Syx (synectin-binding guanine exchange factor; aa 375-792 from accession number NP_001036128.1; DNA for residues 375-392 was generated by oligonucleotides using optimal codons).
Confers ampicillin-resistance.
Backbone vector is pET His6 MBP TEV LIC (2M-T) Addgene # 29708.
This plasmid is mentioned in Fig. S3 of the 2021 bioRxiv paper.
Construct design by Ryan Boyd and Debra T. Hansen.
Cloning by Felicia M. Craciunescu.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179291 Copy
Species: Mus musculus
Genetic Insert: anti-Gephyrin (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2895693. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182100 Copy
Species: Caenorhabditis elegans
Genetic Insert: array co-injection fragment mosTI Pmlc-2_GFP
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159836 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5523; Vector Backbone:pET GFP LIC (2GFP-T) Addgene # 29716; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639414
Comments: Protein sequence is MKSS + HHHHHH + GSS + GFP (aa 3-238 of AGM20710.1 with 4 residue differences) + GIE + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + N + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1).
Confers ampicillin-resistance.
Backbone vector is pET GFP LIC (2GFP-T) Addgene # 29716.
Construct design by Ryan Boyd and Debra T. Hansen.
Cloning by Felicia M. Craciunescu.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179289 Copy
Species: Caenorhabditis elegans
Genetic Insert: hsf-1
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177786 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI NeoR target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159830 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgRNA mosTI neoR
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159831 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5109; Vector Backbone:pET His6 Sumo TEV LIC (2S-T) Addgene # 29711; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639414
Comments: Protein sequence is MKSS + HHHHHH + GSSMAS + SUMO (aa 1-98 of NP_010798.1) + IE + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + N + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1).
Confers ampicillin-resistance.
Backbone vector is pET His6 Sumo TEV LIC (2S-T) Addgene # 29711.
Construct design by Ryan Boyd and Debra T. Hansen.
Cloning by Felicia M. Craciunescu.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179288 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5900; Vector Backbone:pET MBP His6 LIC (2Cc-T), Addgene # 37237; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639414
Comments: Protein sequence is Met + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1) + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + GSSGSM + maltose binding protein (aa 27-393 of AAB59056) + GSS + HHHHHH.
Confers ampicillin-resistance.
Backbone vector is pET MBP His6 LIC (2Cc-T) Addgene # 37237.
Construct design by Ryan Boyd and Debra T. Hansen.
Cloning by Felicia M. Craciunescu.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179286 Copy
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