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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMSCVneo-DEST Resource Report Resource Website |
RRID:Addgene_119746 | Chloramphenicol and Ampicillin | PMID:26595769 | Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:pMSCVneo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-24 02:04:13 | 0 | ||||
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pMSCVhyg-DEST Resource Report Resource Website |
RRID:Addgene_119747 | Chloramphenicol and Ampicillin | Backbone Marker:Clontech; Backbone Size:7000; Vector Backbone:pMSCVhyg; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-24 02:04:13 | 0 | |||||
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pBMN iRFP670-OPTN(F178A/D474N) Resource Report Resource Website |
RRID:Addgene_119694 | OPTN | Homo sapiens | Ampicillin | PMID:30679426 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | F178A/D474N | Addgene | 2026-10-24 02:04:12 | 0 | |
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pCAG PSAM4 GlyR IRES EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_119739 | PSAM4 GlyR IRES eGFP | Homo sapiens | Ampicillin | PMID:30872534 | Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | L131G, Q139L, Y217F | Addgene | 2026-10-24 02:04:13 | 7 | |
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39R861+ Resource Report Resource Website |
RRID:Addgene_119737 | None | PMID:30738800 | Strain can be grown on LB without antibiotics. Resistant to: ampicillin, chloramphenicol, florfenicol, gentamicin, kanamycin, nalidixic acid, streptomycin, spectinomycin, sulphonamides, tobramycin, tetracycline, trimethoprim Please note that plasmids are stable in the absence of antibiotic selection. 39R861+ is resistant to nalidixic acid due to a chromosomal mutation. The remaining resistance determinants are plasmid-borne. 39R861+ contains six plasmids, outlined in the supplemental files. | Vector Backbone:See supplemental files for plasmid details; Vector Types:; Bacterial Resistance:None | Addgene | 2026-10-24 02:04:13 | 0 | |||
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Myc-gamma2/deltaILTM4 Resource Report Resource Website |
RRID:Addgene_119735 | GABRG2/GABRD | Rattus norvegicus | Ampicillin | PMID:22711532 | Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:04:13 | 0 | ||
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pET-NEMOL329P Resource Report Resource Website |
RRID:Addgene_11969 | NEMOL329P | Homo sapiens | Kanamycin | PMID:16547522 | Backbone Size:5370; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Leucine329 to Proline | Addgene | 2026-10-24 02:04:12 | 0 | |
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pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V Resource Report Resource Website 1+ mentions |
RRID:Addgene_119723 | CaMPARI2 | Synthetic | Ampicillin | PMID:30361563 | Backbone Marker:Stratagene; Backbone Size:4267; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | F391W, L398V | Addgene | 2026-10-24 02:04:13 | 1 | |
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mOrange-imp α Resource Report Resource Website |
RRID:Addgene_119720 | importinα | Homo sapiens | Kanamycin | PMID:22313767 | pmOrange-impα was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp α (Addgene plasmid 119679) with mOrange derived from pRSET-B-mOrange (provided by Professor Roger Y. Tsien, UCSD). | Backbone Marker:not commercially available one. Note that Eco47III, AgeI, BspE1 are missing; Vector Backbone:pmOrange-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | contains amino acids 251-529 | Addgene | 2026-10-24 02:04:13 | 0 |
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pBMN mCherry-OPTN(D474N) Resource Report Resource Website |
RRID:Addgene_119683 | OPTN | Homo sapiens | Ampicillin | PMID:30679426 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | D474N | Addgene | 2026-10-24 02:04:12 | 0 | |
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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD) Resource Report Resource Website |
RRID:Addgene_119692 | NBR1 | Homo sapiens | Ampicillin | PMID:30679426 | Please Note: The Phenylalanine to Alanine mutation is at position 563, and the Tyrosine to Alanine mutation is at position 732 relative to the NCBI Reference Sequence NP_005890.2 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | F562A/Y731A & UBD deletion (1-906 ), See depositor comments below | Addgene | 2026-10-24 02:04:12 | 0 |
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pBMN iRFP670-OPTN(D474N) Resource Report Resource Website |
RRID:Addgene_119693 | OPTN | Homo sapiens | Ampicillin | PMID:30679426 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | D474N | Addgene | 2026-10-24 02:04:12 | 0 | |
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pBMN mCherry-NBR1(ΔUBD) Resource Report Resource Website |
RRID:Addgene_119691 | NBR1 | Homo sapiens | Ampicillin | PMID:30679426 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | UBD deletion (1-906) | Addgene | 2026-10-24 02:04:12 | 0 | |
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FB013 Resource Report Resource Website |
RRID:Addgene_119710 | Negative selection marker HSVtk (Pgpda:HSVtk:Ttub) | Cochliobolus heterostrophus (Pgpda), Herpes simplex virus (HSVtk), Neurospora crassa (Ttub) | Chloramphenicol | PMID:29680684 | Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol | Addgene | 2026-10-24 02:04:12 | 0 | ||
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pmCherry-imp α Resource Report Resource Website |
RRID:Addgene_119719 | importinα | Homo sapiens | Kanamycin | PMID:22313767 | pmCherry-impα was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp α (Addgene plasmid 119679) with mCherry derived from pRSET-B-mCherry (provided by Professor Roger Y. Tsien, UCSD). | Backbone Marker:not commercially available one. Note that Eco47III, AgeI, BspE1 are missing.; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | contains amino acids 251-529 | Addgene | 2026-10-24 02:04:13 | 0 |
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FB039 Resource Report Resource Website |
RRID:Addgene_119715 | PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→) | Spectinomycin | PMID:29680684 | Backbone Marker:Diego Orzaez; Backbone Size:7295; Vector Backbone:pDGB3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | Addgene | 2026-10-24 02:04:12 | 0 | |||
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FB030 Resource Report Resource Website |
RRID:Addgene_119714 | Terminator paf | Penicillium chrysogenum | Chloramphenicol | PMID:29680684 | Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol | Addgene | 2026-10-24 02:04:12 | 0 | ||
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pBMN mEGFP-OPTN(F178A) Resource Report Resource Website |
RRID:Addgene_119681 | OPTN | Homo sapiens | Ampicillin | PMID:30679426 | Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | F178A | Addgene | 2026-10-24 02:04:12 | 0 | |
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eGFP-proα2(I)-G610C Resource Report Resource Website 1+ mentions |
RRID:Addgene_119827 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These plasmids were constructed after the G610C mouse model of OI in which the Gly610 residue (starting from the N-terminal end of the triple helix) was substituted with Cys. These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-5 replaced by fluorescent tag, Glycine 610 mutated to Cysteine | Addgene | 2026-10-24 02:04:14 | 2 |
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CP1300-ABEmax Resource Report Resource Website |
RRID:Addgene_119810 | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) | Ampicillin | PMID:31110355 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-24 02:04:13 | 0 |
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