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Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = cysE
Precursor strain = C43(DE3)
Selective amino acid labeling (and/or requirement) = Cys
Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_61953 Copy
Species: Homo sapiens
Genetic Insert: MLH1:JMC01L-F09:C205690
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28a-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4P7A/
Proper citation: RRID:Addgene_61954 Copy
Genetic Insert: MS4A1
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22990521
Proper citation: RRID:Addgene_62009 Copy
Species: Homo sapiens
Genetic Insert: CDYL2
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28a-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://www.thesgc.org/structures/2MJ8/
https://www.thesgc.org/structures/6V2D/
https://www.thesgc.org/structures/6V3N/
Proper citation: RRID:Addgene_61956 Copy
Species: Homo sapiens
Genetic Insert: HRMT1L3:APC035-H01:C21507
Vector Backbone Description: Vector Backbone:pET28a-LIC (Addgene #26094); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: For more information, please see:
PDB / SGC
http://www.thesgc.org/structures/4QQN/
http://www.thesgc.org/structures/3SMQ/
http://www.thesgc.org/structures/4RYL/
https://www.rcsb.org/structure/8G2F/
Proper citation: RRID:Addgene_61951 Copy
Species: Homo sapiens
Genetic Insert: C17orf32
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20693407
Proper citation: RRID:Addgene_62006 Copy
Species: Homo sapiens
Genetic Insert: UBH14
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28a-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4TVR/
Proper citation: RRID:Addgene_61952 Copy
Species: Homo sapiens
Genetic Insert: Tmem38A
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20876400
Proper citation: RRID:Addgene_62007 Copy
Species: Plasmodium falciparum
Genetic Insert: PF08_0034:MAC04J-B11:C229648
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26093; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: SGC links
4QNS http://www.thesgc.org/structures/4QNS/
5TPX http://www.thesgc.org/structures/5TPX/
Proper citation: RRID:Addgene_61957 Copy
Species: Leishmania donovani
Genetic Insert: LdBPK_331470.1:MAC04E-A10:C228347
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26093; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/4QNY/
Proper citation: RRID:Addgene_61958 Copy
Species: Plasmodium falciparum
Genetic Insert: PP-CDPK4
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: http://www.thesgc.org/structures/4QOX/
Proper citation: RRID:Addgene_61959 Copy
Species: Homo sapiens
Genetic Insert: TAPBPL
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20693407
Proper citation: RRID:Addgene_62000 Copy
Species: Homo sapiens
Genetic Insert: NKP90
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20693407
Proper citation: RRID:Addgene_62001 Copy
Species: Homo sapiens
Genetic Insert: HRMT1L6:PBC001-A12:C213642
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid# 62304); Backbone Size:6767; Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: For more information, please see:
http://www.thesgc.org/structures/4QPP/
http://www.thesgc.org/structures/5E8R/
http://www.thesgc.org/structures/5HZM/
http://www.thesgc.org/structures/5WCF/
http://www.thesgc.org/structures/4QQK/
https://www.thesgc.org/structures/6P7I/
Proper citation: RRID:Addgene_61964 Copy
Species: Other
Genetic Insert: N-mCherry-ME(pS)VD
Vector Backbone Description: Backbone Size:3937; Vector Backbone:pNAS1B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25272187
Proper citation: RRID:Addgene_61966 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = cysE hisG
Precursor strain = YM138
modified from the parent Escherichia coli C43(DE3) strain
Selective amino acid labeling (and/or requirement) = Cys, His
Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_61960 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26577727
Comments: Genotype = glyA
Precursor strain = BL21 CodonPlus (DE3)-RIL
Selective amino acid labeling (and/or requirement) = Gly
Each target gene was deleted from the chromosome of BL21 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
This work is supported in part by JSPS-NSF International Collaborations in Chemistry (ICC) research grant.
Proper citation: RRID:Addgene_61962 Copy
Species: Homo sapiens
Genetic Insert: NET30
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21444689
Proper citation: RRID:Addgene_62011 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62147 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62148 Copy
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