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Species: Homo sapiens
Genetic Insert: ALK Receptor Tyrosine Kinase
Vector Backbone Description: Backbone Size:7877; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29650534
Proper citation: RRID:Addgene_183830 Copy
Species: Mus musculus
Genetic Insert: anti-VAChT (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2750807. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182117 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ultra-pan-neuronal positive control pUNP::gfp(NLS)
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist-Amp-high-copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: www.wormbuilder.org
MOdular Safe harbor Transgene Insertion (mosTI).
Positive control vector: P_UPN::gfp(NLS)::tbb-2 UTR
Will give unc-119 rescue and neuronal nuclear GFP expression after insertion into any unc-119 mosTI sites.
See Yemini et al. 2021 Cell for development and characterization of the ultra-pan-neuronal promoter.
Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182359 Copy
Species: Mus musculus
Genetic Insert: anti-Miro2 (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2895710. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182114 Copy
Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExogron Rab11b knock in. DExogron = DExCon (Doxycycline-mediated endogenous gene Expression Control) combined with auxin-mediated targeted protein degradation. Lentiviral delivery of AtAFBP2 and TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179904 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182682 Copy
Species: Oryza sativa
Genetic Insert: osTIR1
Vector Backbone Description: Backbone Size:3045; Vector Backbone:pIVT; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31299080
Proper citation: RRID:Addgene_182043 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab11a knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
AntiGFPnanobody can be used to manipulate with endogenously expressed protein.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179902 Copy
Species: Methanosarcina mazei
Genetic Insert: codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182287 Copy
Species: Synthetic
Genetic Insert: EBFP2-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184461 Copy
Species: Rattus norvegicus
Genetic Insert: iLID mCherry iTrkA
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32335036
Proper citation: RRID:Addgene_136509 Copy
Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179900 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182686 Copy
Species: Mus musculus
Genetic Insert: mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182685 Copy
Species: Aegilops sharonensis
Genetic Insert: pGGG_Sr-1644-1Sh
Vector Backbone Description: Backbone Marker:Hayta et al (2019) https://doi.org/10.1186/s13007-019-0503-z; Backbone Size:5722; Vector Backbone:pGolden Green Gate; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338132
Proper citation: RRID:Addgene_164087 Copy
Species: Homo sapiens
Genetic Insert: SHP2
Vector Backbone Description: Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_8381 Copy
Species: Rattus norvegicus
Genetic Insert: iLID mRuby3 iTrkA
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32335036
Proper citation: RRID:Addgene_136510 Copy
Species: Homo sapiens
Genetic Insert: hM3D (Gq)
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:2900; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35443152
Proper citation: RRID:Addgene_184753 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182661 Copy
Species: Homo sapiens
Genetic Insert: HOTTIP
Vector Backbone Description: Backbone Marker:Benjamin Ebert LAB; Backbone Size:7408; Vector Backbone:pLKO5.sgRNA.EFS.GFP; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31786140
Proper citation: RRID:Addgene_134989 Copy
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