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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pBCN40-R4R3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_34915 | Chloramphenicol and Ampicillin | PMID:22290182 | Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector contains a Pmyo-2::mCherry::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN41-R4R3 which has a green fluorescent pharyngeal marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm). | Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:55 | 4 | |||
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pCFJ448 - pDESTR4-R3(ttTi4348, I, unc-18) Resource Report Resource Website |
RRID:Addgene_34865 | ttTi4348 targeting | Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:22290181 | Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products. | Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:14:03 | 0 | |
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pCFJ210 - pDESTR4-R3(ttTi4348, I) Resource Report Resource Website |
RRID:Addgene_34863 | ttTi4348 targeting | Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:22290181 | Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products. | Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:55 | 0 | |
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pBCN45-R4R3 Resource Report Resource Website |
RRID:Addgene_34920 | Chloramphenicol and Ampicillin | PMID:22290182 | New version of Neomycin resistance vector for drug selection in worms. This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm). | Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:55 | 0 | |||
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pBID-UASC-GRM Resource Report Resource Website 1+ mentions |
RRID:Addgene_35203 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:58 | 1 | |||
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pBID-UASC-G Resource Report Resource Website 1+ mentions |
RRID:Addgene_35202 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11145; Vector Backbone:pBID-UASC-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:14:05 | 6 | |||
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pBID-UASC-FG Resource Report Resource Website 1+ mentions |
RRID:Addgene_35201 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of Flag epitope fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). 3 copies of the Flag epitope is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11240; Vector Backbone:pBID-UASC-FG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:58 | 2 | |||
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pBID-UASC-VG Resource Report Resource Website 1+ mentions |
RRID:Addgene_35206 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-VG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:58 | 2 | |||
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pBID-UASC-GV Resource Report Resource Website 1+ mentions |
RRID:Addgene_35204 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the C-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-GV; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:58 | 2 | |||
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pBID-G Resource Report Resource Website 1+ mentions |
RRID:Addgene_35195 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:10033; Vector Backbone:pBID-G; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:14:04 | 2 | |||
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pBID-UAS-GGi Resource Report Resource Website |
RRID:Addgene_35199 | Chloramphenicol and Ampicillin | PMID:22848718 | Generation of ϕC31 transgenic Drosophila for GAL4 driven RNA inhibition (RNAi). 2 inverted gateway cloning cassettes are flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, hsp70 basal promoter. ftz intron facilitates RNA hairpin formation and expression. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please note that the inverted sequences present can cause rearrangements of this vector. Recommend miniprep only and confirm plasmid map subsequent to amplification or cloning. After gateway recombination, recommend amplification in Stbl2 cells [Invitrogen Cat. 10268-019] and combining minipreps for Drosophila injection. Addgene's sequencing results indicated a single nucleotide mismatch at bp# 5286 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect plasmid function. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102 | Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:13081; Vector Backbone:pBID-UAS-GGi; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:14:05 | 0 | |||
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pCSDest Resource Report Resource Website 10+ mentions |
RRID:Addgene_22423 | Chloramphenicol and Ampicillin | PMID:17948311 | pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette | Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:12 | 17 | |||
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pCSDest2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_22424 | Chloramphenicol and Ampicillin | PMID:17948311 | pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3 | Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:11 | 7 | |||
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pLVU/myc Resource Report Resource Website |
RRID:Addgene_24179 | Chloramphenicol resistance gene (CmR) - ccdB gene | Chloramphenicol resistance gene (CmR) - ccdB gene | Chloramphenicol and Ampicillin | V5 tag deleted | Backbone Marker:Invitrogen; Backbone Size:9400; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:28 | 0 | ||
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pRosa26 R1-ccdB-R2 RexNeo PI-SceI Resource Report Resource Website |
RRID:Addgene_24418 | Chloramphenicol and Ampicillin | PMID:21375737 | Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette. Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418 | Backbone Marker:Soriano Lab (Addgene Plasmid# 21714); Backbone Size:9846; Vector Backbone:pRosa26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:30 | 0 | |||
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pHPdestVenus Resource Report Resource Website |
RRID:Addgene_24568 | reporter gene Venus | Chloramphenicol and Ampicillin | PMID:20506180 | AmpR in backbone; CmR in gateway RfA cassette | Backbone Marker:Scott Barolo; Backbone Size:10474; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:33 | 0 | ||
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pHPdesteGFP Resource Report Resource Website |
RRID:Addgene_24566 | reporter gene eGFP | Chloramphenicol and Ampicillin | PMID:20506180 | AmpR in backbone; CmR in gateway RfA cassette | Backbone Marker:Scott Barolo; Backbone Size:10444; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:32 | 0 | ||
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pLD-puro-CcVA Resource Report Resource Website 1+ mentions |
RRID:Addgene_24588 | Gateway(TM) cassette | Chloramphenicol and Ampicillin | PMID:20305087 | The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA. pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1 VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII | Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:12:32 | 3 | ||
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pDEST-HemmarR Resource Report Resource Website 1+ mentions |
RRID:Addgene_31222 | CD4-tdTom | Homo sapiens | Chloramphenicol and Ampicillin | PMID:21606367 | Gateway destination vector for cloning any enhancer to drive expression of membrane marker CD4-tdTom The depositing laboratory recommends growing bacteria either on LB plates with 80 ug/ml Carbenicillin or in LB liquid media with 60 ug/ml Carbenicillin (a semi-synthetic ampicillin analog) | Backbone Size:10193; Vector Backbone:pAPIC-HIH; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | Fusion of signal peptide of Drosophila Akh gene, human CD4 transmembrane domain, tdTomato, and Kir2.1 ER exit signal. | 2026-08-15 01:13:33 | 2 |
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LZRS-Rfa Resource Report Resource Website 1+ mentions |
RRID:Addgene_31601 | Chloramphenicol and Ampicillin | PMID:15342384 | Backbone Marker:Khavari Lab; Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:13:37 | 5 |
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