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Genetic Insert: Lyn-FRB-GFP11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5415; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34020947
Proper citation: RRID:Addgene_181863 Copy
Species: Synthetic
Genetic Insert: GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183400 Copy
Species: Synthetic
Genetic Insert: LgBiT-CaM
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35254056
Proper citation: RRID:Addgene_183401 Copy
Vector Backbone Description: Vector Backbone:pAAV-VTKS3; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_170854 Copy
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_170851 Copy
Species: Synthetic
Genetic Insert: V5-APEX2-NES
Vector Backbone Description: Vector Backbone:pAAV-VTKD3; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178697 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKS4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178730 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKS5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178731 Copy
Species: Synthetic
Genetic Insert: V5-APEX2-NES
Vector Backbone Description: Vector Backbone:pAAV-VTKD2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178696 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178727 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178728 Copy
Vector Backbone Description: Vector Backbone:pAAV-VTKD1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_170846 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178729 Copy
Genetic Insert: M2rtTA-T2A-Puro
Vector Backbone Description: Backbone Marker:Transposagen Bio; Vector Backbone:SPB-007; Vector Types:Mammalian Expression, piggyBac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33596423
Comments: For use in Tet-On inducible expression systems:
The use of two different antibiotic-resistance genes allows for the selection of clones that incorporate the two vectors in a single step. Although all transgene sequences for conditional gene expression could be potentially cloned into a single plasmid, a two plasmid system provides more flexibility and permits the adjustment of the TRE/M2rtTA ratio to optimize hPSC generation with robust DOX-dependent gene expression, while avoiding transgene leakage.
Please note: Plasmid contains an E48K mutation in PuroR. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_169175 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD6; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178723 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKS4; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178724 Copy
Species: Synthetic
Genetic Insert: ChR2-YFP
Vector Backbone Description: Vector Backbone:pAAV-VTKD5; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784651
Proper citation: RRID:Addgene_178722 Copy
Species: Mus musculus
Genetic Insert: anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2895701. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182106 Copy
Species: Synthetic
Genetic Insert: Nuclease-dead Lb Cas12a
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35414015
Proper citation: RRID:Addgene_183955 Copy
Species: Mus musculus
Genetic Insert: anti-Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2895699. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_182103 Copy
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