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ML43
 
Resource Report
Resource Website 		RRID:Addgene_61922 none None PMID:21925267 Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB Precursor strain = ML42 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr, Asn *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. Vector Backbone:none; Vector Types:; Bacterial Resistance:None 2026-08-15 01:17:48 0
pFA6a LEU2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61923 LEU2 Kluyveromyces lactis Ampicillin PMID:21768125 Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 1
pVITRO1-Trastuzumab-IgG1/κ
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_61883 HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette Homo sapiens Hygromycin PMID:25073855 Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:17:47 20
pVITRO1-Trastuzumab-IgG3/κ
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61885 HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette Homo sapiens Hygromycin PMID:25073855 Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:17:47 1
Rtn4a-GFP
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_61807 Rtn4a Homo sapiens Kanamycin PMID:18442980 This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Rtn4a Stop Codon removed 2026-08-15 01:17:46 14
pET151-mUCH37 (ISF2)
 
Resource Report
Resource Website 		RRID:Addgene_61928 UCH37 (M.musculus) Mus musculus Ampicillin PMID:25702872 Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 0
pET151-hUCH37 (ISF1)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61929 hUCH37 (ISF1) Homo sapiens Ampicillin PMID:25702872 Backbone Marker:Invotrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:48 2
pCMV-Ubl4A K48R
 
Resource Report
Resource Website 		RRID:Addgene_61809 UBL4A Homo sapiens Kanamycin PMID:24424410 Backbone Marker:Origene; Backbone Size:4929; Vector Backbone:pCMV6-ENTRY; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin K48R 2026-08-15 01:17:46 0
pRS316 GAL cluster
 
Resource Report
Resource Website 		RRID:Addgene_61925 GAL1 Saccharomyces cerevisiae Ampicillin PMID:24520161 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:48 0
pMU102
 
Resource Report
Resource Website 		RRID:Addgene_61805 Chloramphenicol PMID:22578246 Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:46 0
pEGFP-AID
 
Resource Report
Resource Website 		RRID:Addgene_61926 AICDA Homo sapiens Kanamycin PMID:24324754 Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:47 0
pVITRO1-102.1F10-IgM/λ
 
Resource Report
Resource Website 		RRID:Addgene_61880 grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette Homo sapiens Hygromycin PMID:25073855 Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:17:47 0
px330-USP13
 
Resource Report
Resource Website 		RRID:Addgene_61812 USP13 Homo sapiens Ampicillin PMID:24424410 Because there is no selective marker in the vector, Western Blot should be used to screen the knockout clones. Backbone Marker:Addgene; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
pSARSF53-110
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61813 TvoVMAN11 intein Thermoplasma volcanium Kanamycin PMID:24574026 Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:47 1
pAD06 K. lactis telomerase RNA hairpin
 
Resource Report
Resource Website 		RRID:Addgene_61895 Telomerase RNA hairpin (nt 229-273) Kluyveromyces lactis Ampicillin PMID:23610127 FokI site follows RNA construct to linearize plasmid prior to in vitro transcription. Backbone Size:2686; Vector Backbone:Puc19; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 0
pET151-hUCH37 (ISF2)
 
Resource Report
Resource Website 		RRID:Addgene_61930 hUCH37 (ISF1) Homo sapiens Ampicillin PMID:25702872 Isoform 2 was created by deletion of E246 by Quickchange mutagenesis from pET151-hUCH37 (ISF1) vector. Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 0
pSARSF53-165
 
Resource Report
Resource Website 		RRID:Addgene_61817 PhoRadAdC6 intein Pyrococcus horikoshii Kanamycin PMID:24574026 Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:46 0
pAD04 S. arboricola telomerase RNA KBS
 
Resource Report
Resource Website 		RRID:Addgene_61893 Telomerase RNA hairpin Saccharomyces arboricola Ampicillin PMID:23610127 FokI site follows RNA construct to linearize plasmid prior to in vitro transcription. Backbone Size:2686; Vector Backbone:Puc19; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:17:47 0
pSABAD14-111
 
Resource Report
Resource Website 		RRID:Addgene_61824 NpuDnaB(delta283)dN11 intein Nostoc punctiforme Ampicillin PMID:24574026 Backbone Marker:invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
pcDNA3-GFP-LplA(AAG)
 
Resource Report
Resource Website 		RRID:Addgene_61820 E. coli lipoic acid ligase Ampicillin PMID:25313043 Backbone Size:5460; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E20A, F147A, H149G 2026-08-15 01:17:46 0

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