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Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:21925267
Comments: Genotype = cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB
Precursor strain = ML42
modified from the parent Escherichia coli C43(DE3) strain
Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys, Thr, Asn
*In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)].
#In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)].
Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system.
Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.
Proper citation: RRID:Addgene_61922 Copy
Species: Kluyveromyces lactis
Genetic Insert: LEU2
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21768125
Proper citation: RRID:Addgene_61923 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61883 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61885 Copy
Species: Homo sapiens
Genetic Insert: Rtn4a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18442980
Comments: This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_61807 Copy
Species: Mus musculus
Genetic Insert: UCH37 (M.musculus)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702872
Proper citation: RRID:Addgene_61928 Copy
Species: Homo sapiens
Genetic Insert: hUCH37 (ISF1)
Vector Backbone Description: Backbone Marker:Invotrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702872
Proper citation: RRID:Addgene_61929 Copy
Species: Homo sapiens
Genetic Insert: UBL4A
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4929; Vector Backbone:pCMV6-ENTRY; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24424410
Proper citation: RRID:Addgene_61809 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24520161
Proper citation: RRID:Addgene_61925 Copy
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22578246
Proper citation: RRID:Addgene_61805 Copy
Species: Homo sapiens
Genetic Insert: AICDA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24324754
Proper citation: RRID:Addgene_61926 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61880 Copy
Species: Homo sapiens
Genetic Insert: USP13
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Comments: Because there is no selective marker in the vector, Western Blot should be used to screen the knockout clones.
Proper citation: RRID:Addgene_61812 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAN11 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61813 Copy
Species: Kluyveromyces lactis
Genetic Insert: Telomerase RNA hairpin (nt 229-273)
Vector Backbone Description: Backbone Size:2686; Vector Backbone:Puc19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23610127
Comments: FokI site follows RNA construct to linearize plasmid prior to in vitro transcription.
Proper citation: RRID:Addgene_61895 Copy
Species: Homo sapiens
Genetic Insert: hUCH37 (ISF1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pET151-D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25702872
Comments: Isoform 2 was created by deletion of E246 by Quickchange mutagenesis from pET151-hUCH37 (ISF1) vector.
Proper citation: RRID:Addgene_61930 Copy
Species: Pyrococcus horikoshii
Genetic Insert: PhoRadAdC6 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61817 Copy
Species: Saccharomyces arboricola
Genetic Insert: Telomerase RNA hairpin
Vector Backbone Description: Backbone Size:2686; Vector Backbone:Puc19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23610127
Comments: FokI site follows RNA construct to linearize plasmid prior to in vitro transcription.
Proper citation: RRID:Addgene_61893 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaB(delta283)dN11 intein
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61824 Copy
Genetic Insert: E. coli lipoic acid ligase
Vector Backbone Description: Backbone Size:5460; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25313043
Proper citation: RRID:Addgene_61820 Copy
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