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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3.1 Flag-Sesn2 Resource Report Resource Website |
RRID:Addgene_61868 | Sesn2 | Mus musculus | Ampicillin | PMID:25259925 | Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:17:46 | 0 | |
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ML2 Resource Report Resource Website |
RRID:Addgene_61906 | none | Kanamycin | PMID:21925267 | Genotype = cyo::kan++ ilvE (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Ile, Leu* CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:47 | 0 | ||
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ML3 Resource Report Resource Website |
RRID:Addgene_61907 | none | Kanamycin | PMID:21925267 | Genotype = cyo::kan hisG (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = His CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:47 | 0 | ||
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ML6 Resource Report Resource Website |
RRID:Addgene_61908 | none | Kanamycin | PMID:21925267 | Genotype = cyo::kan++ ilvE avtA (Kan cassette in the chromosomal DNA) Precursor strain = ML2 (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Ile, Leu*, Val CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:47 | 0 | ||
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ML8 Resource Report Resource Website |
RRID:Addgene_61909 | none | Kanamycin | PMID:21925267 | Genotype = cyo::kan argH (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Arg CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:47 | 0 | ||
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pcDNA3.1 Flag-Sesn3 Resource Report Resource Website |
RRID:Addgene_61869 | Sesn3 | Mus musculus | Ampicillin | PMID:25259925 | Backbone Size:6000; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:17:47 | 0 | |
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ML12 Resource Report Resource Website |
RRID:Addgene_61910 | none | Kanamycin | PMID:21925267 | Genotype = cyo::kan ilvE avtA aspC (Kan cassette in the chromosomal DNA) Precursor strain = ML6 (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur (see Tables 2, 3). Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:47 | 0 | ||
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pVITRO1-dV-IgE/κ Resource Report Resource Website |
RRID:Addgene_61878 | Human immunoglobulin Epsilon constant region | Homo sapiens | Hygromycin | PMID:25073855 | Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin | Deleted Variable region | 2026-08-15 01:17:47 | 0 | |
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pVITRO1-dV-IgE/λ Resource Report Resource Website |
RRID:Addgene_61879 | Human immunoglobulin Epsilon constant region | Homo sapiens | Hygromycin | PMID:25073855 | Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin | Deleted Variable region | 2026-08-15 01:17:47 | 0 | |
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pGEX-6p-1 Sesn1b Resource Report Resource Website |
RRID:Addgene_61874 | Sesn1 | Mus musculus | Ampicillin | PMID:25259925 | Backbone Size:6000; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:17:47 | 0 | |
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ML26 Resource Report Resource Website |
RRID:Addgene_61917 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH Precursor strain = ML23 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:47 | 0 | ||
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ML31 Resource Report Resource Website |
RRID:Addgene_61918 | none | None | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA Precursor strain = ML26 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:47 | 0 | ||
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pFa6a MET25 Resource Report Resource Website |
RRID:Addgene_61913 | MET25 | Saccharomyces cerevisiae | Ampicillin | The amplified region is functional, but does not overlap with the MET25 deletion region in BY4741 and derivatives, so will not recombine at the endogenous locus in this background. | Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:47 | 0 | ||
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ML21 Resource Report Resource Website |
RRID:Addgene_61914 | none | None | PMID:21925267 | Genotype = tyrA hisG Precursor strain = ML14 modified from the parent Escherichia coli C43(DE3) strain Selective amino acid labeling (and/or requirement) = (Tyr), His Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:17:47 | 0 | ||
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pInducer21 Flag-Sesn2 419A/422A/426A Resource Report Resource Website |
RRID:Addgene_61870 | Sesn2 | Mus musculus | Ampicillin | PMID:25259925 | Backbone Size:13000; Vector Backbone:pInducer21; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 419A/422A/426A | 2026-08-15 01:17:47 | 0 | |
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pVITRO1-Trastuzumab-IgG4/κ Resource Report Resource Website 1+ mentions |
RRID:Addgene_61887 | HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette | Homo sapiens | Hygromycin | PMID:25073855 | Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:17:47 | 2 | ||
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ML40K1 Resource Report Resource Website |
RRID:Addgene_61920 | none | Chloramphenicol | PMID:21925267 | Genotype = cyo ilvE avtA aspC hisG argH metA lysA Precursor strain = ML31 Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys ML40(K1) contains an extra pACYC plasmid carrying the cat cassette plus extra tRNA genes (argU, ileY, leuW) from Agilent Technologies Inc. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. | Vector Backbone:none; Vector Types:; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:17:47 | 0 | ||
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pVITRO1-Trastuzumab-IgA1/κ Resource Report Resource Website |
RRID:Addgene_61888 | HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette | Homo sapiens | Hygromycin | PMID:25073855 | Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:17:47 | 0 | ||
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GFP-Rab4B Resource Report Resource Website |
RRID:Addgene_61801 | Rab4B | Homo sapiens | Kanamycin | PMID:25416943 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | None | 2026-08-15 01:17:46 | 0 | |
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pVITRO1-Trastuzumab-IgA2/κ Resource Report Resource Website |
RRID:Addgene_61889 | HER2/neu receptor specific humanized Alpha 2 heavy chain expression cassette | Homo sapiens | Hygromycin | PMID:25073855 | Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin | 2026-08-15 01:17:47 | 0 |
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