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Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Comments: Addgene QC NGS identifies discrepancies relative to the pRK5 backbone, including the addition of NeoR.
Proper citation: RRID:Addgene_61742 Copy
Species: Homo sapiens
Genetic Insert: USP13/5-1 chimera
Vector Backbone Description: Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Proper citation: RRID:Addgene_61743 Copy
Species: Homo sapiens
Genetic Insert: USP13/5-6 chimera
Vector Backbone Description: Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Comments: mutation D23Y in USP13 does not affect plasmid function
Proper citation: RRID:Addgene_61748 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaEN35 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61755 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAdC48 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61759 Copy
Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:PET42C(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24424410
Proper citation: RRID:Addgene_61750 Copy
Species: Streptomyces hygroscopicus
Genetic Insert: Bar gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCAMB-BAR (pCAMBIA-0380 backbone with bar gene under Neurospora crassa trpC promoter) For more information see: Kramer et al.. Fungal Genetics and Biology, 46, 667-681. https://www.ncbi.nlm.nih.gov/pubmed/19520179.
Bar gene from pCAMB-BAR was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-BAR-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and bar sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61766 Copy
Species: E. coli
Genetic Insert: nptII gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter).
NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp).
Proper citation: RRID:Addgene_61767 Copy
Species: MS2 virus
Genetic Insert: MS2 coat protein fused to eGFP
Vector Backbone Description: Backbone Size:4781; Vector Backbone:Lentiviral expression vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25271374
Proper citation: RRID:Addgene_61764 Copy
Species: Synthetic
Genetic Insert: Hygromycin
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61765 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAdC7 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61761 Copy
Species: Mus musculus
Genetic Insert: mouse Smad5 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Comments: Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits
Proper citation: RRID:Addgene_61777 Copy
Species: Mus musculus
Genetic Insert: mouse Xbp1 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Comments: Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits
Proper citation: RRID:Addgene_61779 Copy
Species: Mus musculus
Genetic Insert: mouse Smad2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61775 Copy
Species: Mus musculus
Genetic Insert: mouse Map3k1 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Comments: Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits
Proper citation: RRID:Addgene_61776 Copy
Species: Mus musculus
Genetic Insert: mouse Zeb2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61771 Copy
Species: Mus musculus
Genetic Insert: mouse Crtap 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61772 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7988; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61702 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61703 Copy
Species: Mus musculus
Genetic Insert: mouse Skp2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25798844
Proper citation: RRID:Addgene_61784 Copy
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