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Plasmids are provided by Addgene and DGRC.

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pRK5-FLAG-USP5
 
Resource Report
Resource Website 		RRID:Addgene_61742 USP5 Homo sapiens Ampicillin PMID:24424410 Addgene QC NGS identifies discrepancies relative to the pRK5 backbone, including the addition of NeoR. Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:17:45 0
PRK5-FLAG-USP13/5-1
 
Resource Report
Resource Website 		RRID:Addgene_61743 USP13/5-1 chimera Homo sapiens Ampicillin PMID:24424410 Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin USP5 (1-185aa) fused to USP13 (197-864aa) 2026-08-15 01:17:46 0
PRK5-FLAG-USP13/5-6
 
Resource Report
Resource Website 		RRID:Addgene_61748 USP13/5-6 chimera Homo sapiens Ampicillin PMID:24424410 mutation D23Y in USP13 does not affect plasmid function Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin USP13 (1-721aa) fused to USP5 (717-859aa); D23Y in USP13-please see depositor comment below 2026-08-15 01:17:45 0
pSARSF53-218
 
Resource Report
Resource Website 		RRID:Addgene_61755 NpuDnaEN35 intein Nostoc punctiforme Kanamycin PMID:24574026 Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:46 0
pSARSF53-343
 
Resource Report
Resource Website 		RRID:Addgene_61759 TvoVMAdC48 intein Thermoplasma volcanium Kanamycin PMID:24574026 Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:45 0
pet42c(+)-GST-USP5
 
Resource Report
Resource Website 		RRID:Addgene_61750 USP5 Homo sapiens Kanamycin PMID:24424410 Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:PET42C(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:45 0
pC-BAR-YR
 
Resource Report
Resource Website 		RRID:Addgene_61766 Bar gene Streptomyces hygroscopicus Kanamycin PMID:26092796 Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCAMB-BAR (pCAMBIA-0380 backbone with bar gene under Neurospora crassa trpC promoter) For more information see: Kramer et al.. Fungal Genetics and Biology, 46, 667-681. https://www.ncbi.nlm.nih.gov/pubmed/19520179. Bar gene from pCAMB-BAR was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-BAR-YR. Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and bar sequences are present within the EcoRI and HindII cloning sites. See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning. Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin 2026-08-15 01:17:45 0
pC-G418-YR
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61767 nptII gene E. coli Kanamycin PMID:26092796 Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter). NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR. Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites. See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning. Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp). Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin 2026-08-15 01:17:46 1
MS2_GFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61764 MS2 coat protein fused to eGFP MS2 virus Ampicillin PMID:25271374 Backbone Size:4781; Vector Backbone:Lentiviral expression vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 3
pC-HYG-YR
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61765 Hygromycin Synthetic Kanamycin PMID:26092796 Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561. Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites. See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning. Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin 2026-08-15 01:17:45 1
pSARSF53-HY176
 
Resource Report
Resource Website 		RRID:Addgene_61761 TvoVMAdC7 intein Thermoplasma volcanium Kanamycin PMID:24574026 Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:46 0
psiCHECK-2-Smad5-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61777 mouse Smad5 3'UTR Mus musculus Ampicillin PMID:25798844 Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:45 0
psiCHECK-2-Xbp1-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61779 mouse Xbp1 3'UTR Mus musculus Ampicillin PMID:25798844 Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:45 0
psiCHECK-2-Smad2-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61775 mouse Smad2 3'UTR Mus musculus Ampicillin PMID:25798844 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
psiCHECK-2-Map3k1-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61776 mouse Map3k1 3'UTR Mus musculus Ampicillin PMID:25798844 Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:45 0
psiCHECK-2-Zeb2-3'UTR
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_61771 mouse Zeb2 3'UTR Mus musculus Ampicillin PMID:25798844 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:45 1
psiCHECK-2-Crtap-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61772 mouse Crtap 3'UTR Mus musculus Ampicillin PMID:25798844 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0
pGADT7-GW
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_61702 Chloramphenicol and Ampicillin PMID:19843313 Backbone Marker:Clontech; Backbone Size:7988; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:17:45 14
pGBKT7-GW
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_61703 Chloramphenicol and Kanamycin PMID:19843313 Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-15 01:17:45 11
psiCHECK-2-Skp2-3'UTR
 
Resource Report
Resource Website 		RRID:Addgene_61784 mouse Skp2 3'UTR Mus musculus Ampicillin PMID:25798844 Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:17:46 0

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