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Genetic Insert: Nx1BTM-FCS-ChR2ET/TC-mVenus
Vector Backbone Description: Backbone Size:5012; Vector Backbone:AAV2-CAG-MSC-WPRE(Penn); Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30343901
Proper citation: RRID:Addgene_119298 Copy
Genetic Insert: split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119299 Copy
Genetic Insert: PBAD
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: L-arabinose inducible promoter from the arabinose operon in E. coli. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119574 Copy
Species: Synthetic
Genetic Insert: J23108MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119582 Copy
Species: Synthetic
Genetic Insert: J23109MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119583 Copy
Species: Synthetic
Genetic Insert: J23106MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119580 Copy
Species: Synthetic
Genetic Insert: J23107MH
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119581 Copy
Genetic Insert: PrnpB
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: RNase P subunit B promoter. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119569 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Venus was developed by Miyawaki and colleagues. The reference is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90. The EYFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11931 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11930 Copy
Species: Homo sapiens
Genetic Insert: KIAA1524
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28174209
Comments: 522 AGA-> GAT mutation
Proper citation: RRID:Addgene_119289 Copy
Genetic Insert: ParsB
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: AsO2- inducible promoter from the arsB gene. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119565 Copy
Species: Homo sapiens
Genetic Insert: KIAA1524
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28174209
Proper citation: RRID:Addgene_119287 Copy
Species: Synthetic
Genetic Insert: J23104MH
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119562 Copy
Species: Synthetic
Genetic Insert: J23111MH
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119563 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 or pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Addgene Note: Please see the Addgene sequencing result to view the fluorophore sequence.
Proper citation: RRID:Addgene_11937 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11936 Copy
Genetic Insert: PpetE
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Cu2+ inducible promoter from the PetE gene. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Addgene NGS identified potential sequence discrepancies in PpetE, see Genbank file for these annotations.
Proper citation: RRID:Addgene_119572 Copy
Species: Homo sapiens
Genetic Insert: PPME1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26499835
Proper citation: RRID:Addgene_119292 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16606690
Comments: PAGFP is a photoactivatable version of GFP. It is essentially the same as the standard EGFP-C1 from clontech, but contains the mutations L64F/T65S/V163A/T203H, and conatins an A206K mutation to disrupt dimerization.
In total, the PAGFP-Ubiquitin insert is 983bp.
Proper citation: RRID:Addgene_11933 Copy
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