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Genetic Insert: Kana_I-SceI
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16526409
Proper citation: RRID:Addgene_61601 Copy
Vector Backbone Description: Backbone Size:4658; Vector Backbone:pBAMD1-2; Vector Types:Synthetic Biology, Mini-Tn5 vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25389526
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_61564 Copy
Species: horse
Genetic Insert: Latherin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23782536
Proper citation: RRID:Addgene_61685 Copy
Vector Backbone Description: Backbone Size:4730; Vector Backbone:pBAMD1-4; Vector Types:Synthetic Biology, Mini-Tn5 vector; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25389526
Proper citation: RRID:Addgene_61565 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8658; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_61600 Copy
Genetic Insert: LSSmOrange and CNA35
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25490719
Proper citation: RRID:Addgene_61605 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase W235F
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16482214
Proper citation: RRID:Addgene_61681 Copy
Species: Rattus norvegicus
Genetic Insert: NES-jRCaMP1a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27011354
Proper citation: RRID:Addgene_61562 Copy
Species: Rattus norvegicus
Genetic Insert: NES-jRGECO1a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27011354
Proper citation: RRID:Addgene_61563 Copy
Species: Mus musculus
Genetic Insert: IFT88
Vector Backbone Description: Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23930224
Proper citation: RRID:Addgene_61684 Copy
Species: Homo sapiens
Genetic Insert: NAD(P)H dehydrogenase, quinone 1 (NQO1)
Vector Backbone Description: Vector Backbone:pEFIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15687255
Comments: Addgene sequencing results find that the Y to V mutation is at position 129 with respect to reference sequence NP_000894.1
Proper citation: RRID:Addgene_61733 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61736 Copy
Species: Hepatitis C Virus
Genetic Insert: NS4A
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24130685
Comments: For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG
Proper citation: RRID:Addgene_61696 Copy
Species: Pseudomonas stutzeri
Genetic Insert: Opt13
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17615560
Proper citation: RRID:Addgene_61698 Copy
Species: Synthetic
Genetic Insert: sSpCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Apramycin
Defining Citation: PMID:25458909
Proper citation: RRID:Addgene_61737 Copy
Species: Synthetic
Genetic Insert: scTALEN-ABL1-R
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336919
Proper citation: RRID:Addgene_61695 Copy
Species: Francisella tularensis subsp. tularensis
Genetic Insert: enoyl-[acyl-carrier-protein] reductase (NADH)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22098466
Comments: BL21 Gold cells for protein expression
Proper citation: RRID:Addgene_61690 Copy
Species: Staphylococcus aureus subsp. aureus
Genetic Insert: FabI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: BL21 Gold cells for protein expression
Proper citation: RRID:Addgene_61691 Copy
Species: Homo sapiens
Genetic Insert: USP13/5-3 chimera
Vector Backbone Description: Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Proper citation: RRID:Addgene_61745 Copy
Species: Homo sapiens
Genetic Insert: USP13/5-5 chimera
Vector Backbone Description: Backbone Size:5204; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24424410
Comments: G2667A (V570I in USP5) does not affect the function of USP5.
Proper citation: RRID:Addgene_61747 Copy
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