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Genetic Insert: split nTEVp in sufion with PYL1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119213 Copy
Species: Piper methysticum
Genetic Insert: PmCHS
Vector Backbone Description: Backbone Size:6352; Vector Backbone:p426TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119177 Copy
Genetic Insert: cyclic luciferase with SuMMVp cleavage site
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119210 Copy
Species: Piper methysticum
Genetic Insert: Pm4CL1
Vector Backbone Description: Backbone Size:3233; Vector Backbone:pJKW1565; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119178 Copy
Genetic Insert: split nPPVp in fusion with PYL1
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119211 Copy
Species: Homo sapiens
Genetic Insert: human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP]
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5569; Vector Backbone:pSBtet-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30538252
Proper citation: RRID:Addgene_119187 Copy
Genetic Insert: split cSuMMVp in fusion with FKBP
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119182 Copy
Genetic Insert: HyPer3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4600; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279532
Comments: Can be used for in vivo expression of the hydrogen peroxide-sensitive fluorescent protein HyPer3. It was used for this purpose in Steinhorn et al. Free Radic Biol Med. 2017 Dec;113:16-25. doi: 10.1016/j.freeradbiomed.2017.09.006.
In the associated publication (Steinhorn et al. Nat Commun. 2018 Oct 2;9(1):4044. doi: 10.1038/s41467-018-06533-2), it was used as a control for a virus driving expression of a fusion between HyPer and D-amino acid oxidase.
Proper citation: RRID:Addgene_119183 Copy
Species: Piper methysticum
Genetic Insert: Pm4CL1
Vector Backbone Description: Backbone Size:3233; Vector Backbone:pJKW1565; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119180 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 2 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119205 Copy
Species: Homo sapiens
Genetic Insert: sh1miR targeting NF1
Vector Backbone Description: Backbone Marker:Modified from pGIPZ; Backbone Size:9359; Vector Backbone:pLV-H1-SGIPZ; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin and Bleocin (Zeocin)
Defining Citation: PMID:30408279
Comments: The miRNA may also target additional species.
The vector also expresses turboGFP.
The restriction sites useful for verifying the vector and distinguishing from the empty vector (Addgene #119217) are indicated on the map; we suggest using MluI+SalI or MluI+BsrGI. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119206 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 2 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119203 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 1 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments for Addgene #119200. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119204 Copy
Species: Homo sapiens
Genetic Insert: NF1 type 1 mini-gene
Vector Backbone Description: Backbone Marker:Modified from pGEX-4T-1; Backbone Size:2203; Vector Backbone:pAMP-CY1; Vector Types:Transfer vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30408279
Comments: See the attached comments. For proper annotations, see the attached gbk file; the sequence completely matches Addgene NGS results. Plasmid designed and cloned by Yan Cui, PhD
Proper citation: RRID:Addgene_119200 Copy
Genetic Insert: cyclic luciferase with SbMVp cleavage site
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119209 Copy
Genetic Insert: cyclic luciferase with PPVp cleavage site
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531965
Proper citation: RRID:Addgene_119208 Copy
Species: Piper methysticum
Genetic Insert: PmSPS1
Vector Backbone Description: Backbone Size:6352; Vector Backbone:p426TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119175 Copy
Species: Homo sapiens
Genetic Insert: NKX2-1
Vector Backbone Description: Vector Backbone:pMSCV-hygromycin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17925434
Proper citation: RRID:Addgene_119173 Copy
Species: Piper methysticum
Genetic Insert: Pm4CL1
Vector Backbone Description: Backbone Size:7463; Vector Backbone:p425TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119174 Copy
Species: R. gracilis
Genetic Insert: HyPer-DAAO-NES
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4600; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279532
Proper citation: RRID:Addgene_119164 Copy
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