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Vector Backbone Description: Backbone Size:2959; Vector Backbone:pBS SK2+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25741722
Comments: The Zeng lab recommends amplifying this plasmid in 15 microg/ml kanamycin to reduce recombination. Addgene is not able to supply the plasmid under these selection conditions and the stab you receive will be under Amp selection. We recommend using the low conc. Kan selection and checking your plasmid prep for recombination.
Proper citation: RRID:Addgene_61580 Copy
Species: Homo sapiens
Genetic Insert: rhoA
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600581
Proper citation: RRID:Addgene_61632 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7600581
Proper citation: RRID:Addgene_61633 Copy
Species: Staphylococcus aureus
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Proper citation: RRID:Addgene_61593 Copy
Species: Other
Genetic Insert: hSaCas9
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25830891
Comments: Note: This plasmid does not have an sgRNA expression cassette. Please refer to the associated publication, as well as the AAV portion of http://www.addgene.org/crispr/zhang/ , for more information on expressing sgRNAs.
Proper citation: RRID:Addgene_61594 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61649 Copy
Genetic Insert: SNAP-EF(N)-5nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61659 Copy
Species: Auquorea victoria
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61655 Copy
Genetic Insert: SNAP-EF(N)-60nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61663 Copy
Genetic Insert: SNAP-EF(N)-10nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61660 Copy
Genetic Insert: SNAP-EF(N)-30nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61662 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a C terminal Myc tag in frame with pHoran.
Proper citation: RRID:Addgene_61557 Copy
Genetic Insert: mEGFP-30nmSAH-IQ
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61678 Copy
Species: Synthetic
Genetic Insert: pHoran4
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Proper citation: RRID:Addgene_61558 Copy
Genetic Insert: mEGFP-EF(N)-30nmSAH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61679 Copy
Species: Mus musculus
Genetic Insert: Kdm4d
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25417163
Proper citation: RRID:Addgene_61553 Copy
Species: Rattus norvegicus
Genetic Insert: KIF5C(1-560)-EF(C)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61675 Copy
Species: Rattus norvegicus
Genetic Insert: KHC(1-560)-mCitrine
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61676 Copy
Species: Synthetic
Genetic Insert: pHuji
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25385186
Comments: There is a N-terminal HA tag in frame with pHuji.
Proper citation: RRID:Addgene_61556 Copy
Species: Synthetic
Genetic Insert: HIV-1 integrase F185H/C280S
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8631811
Proper citation: RRID:Addgene_61672 Copy
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