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Genetic Insert: SV40 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10373518
Comments: pRL-SV40P was generated by placing the simian virus 40 (SV40) promoter from pGL3-Promoter (Promega) into pRL-TK such that the SV40 promoter is driving the Renilla luciferase gene.
Proper citation: RRID:Addgene_27163 Copy
Species: Nematostella vectensis
Genetic Insert: Nv-NF-kB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19806194
Comments: Clone starts at aa 2.
Proper citation: RRID:Addgene_27260 Copy
Species: Nematostella vectensis
Genetic Insert: Nv-NF-kB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19806194
Comments: Clone starts at aa 2.
Proper citation: RRID:Addgene_27259 Copy
Species: Reticuloendotheliosis virus
Genetic Insert: v-Rel
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_27256 Copy
Species: Homo sapiens
Genetic Insert: REL
Vector Backbone Description: Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14534540
Proper citation: RRID:Addgene_27257 Copy
Species: Mus musculus
Genetic Insert: Trip6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395914
Comments: Trip6 cDNA starts at aa 2.
Proper citation: RRID:Addgene_27254 Copy
Species: Homo sapiens
Genetic Insert: Trip6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10794523
Comments: Trip6 cDNA contains starts at aa 2.
Proper citation: RRID:Addgene_27255 Copy
Species: Homo sapiens
Genetic Insert: REL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18695674
Proper citation: RRID:Addgene_27253 Copy
Species: Homo sapiens
Genetic Insert: prom PDE4D3
Vector Backbone Description: Backbone Size:4370; Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20952536
Proper citation: RRID:Addgene_27304 Copy
Species: Homo sapiens
Genetic Insert: bridging integrator 1 (BIN1), transcript variant 8
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pDEST/N1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20169111
Proper citation: RRID:Addgene_27305 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4985; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18385322
Proper citation: RRID:Addgene_27269 Copy
Species: Homo sapiens
Genetic Insert: prom PDE4D3
Vector Backbone Description: Backbone Size:4370; Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20952536
Proper citation: RRID:Addgene_27302 Copy
Species: Homo sapiens
Genetic Insert: bridging integrator 1 (BIN1), transcript variant 8
Vector Backbone Description: Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-Dest (Invitrogen); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20169111
Proper citation: RRID:Addgene_27303 Copy
Species: Homo sapiens
Genetic Insert: REL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17072339
Comments: Please note that Addgene's sequencing results confirmed N424 is present in the REL sequence. Despite the plasmid name, amino acids 425-490 of REL have been deleted. This plasmid is exactly as created by the depositing laboratory and as described in the associated publication listed below and in Chin et al., Oncogene. 2009 May 21;28(20):2100-11.
Proper citation: RRID:Addgene_27267 Copy
Species: Homo sapiens
Genetic Insert: NEMO
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18164680
Comments: NEMO cDNA starts at aa 2.
Proper citation: RRID:Addgene_27268 Copy
Species: Homo sapiens
Genetic Insert: I-kappa-B kinase epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12692549
Proper citation: RRID:Addgene_27238 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:9941; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15608064
Comments: For rodent cortex pyramidal neuron-specific expression of EGFP under the synapsin I promoter (1.1kb fragment)
Proper citation: RRID:Addgene_27232 Copy
Genetic Insert: DsRed2
Vector Backbone Description: Backbone Size:9066; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15608064
Comments: Expresses tandem dimer RFP under the mouse α-CaMKII promoter (1.3kb fragment). Similar to plasmid # 27230, except for fluorescent tag.
Note that differences between Addgene's sequencing result and the sequence from the depositing scientist are due to use of theoretical maps. Please utilize Addgene's sequence when available.
EcoRI and BstBI sites are not present according to Addgene's sequencing results.
Proper citation: RRID:Addgene_27233 Copy
Species: Danio rerio
Genetic Insert: Zinc finger array targeting hnf1a
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence.
Users must order the complementary plasmid hnf1a_L (OZ521) [Addgene plasmid 27196] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene.
Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203).
This zinc finger array was tested for binding activity to the sequence 5'-GAGGTAGTG-3' in a bacterial two hybrid assay, and resulted in 7.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus).
Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication.
Other Articles:
"Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082
"Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934
Proper citation: RRID:Addgene_27197 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:9941; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15608064
Comments: For rodent cortex pyramidal neuron-specific expression of EGFP under the mouse α-CaMKII promoter (1.3kb fragment)
Proper citation: RRID:Addgene_27230 Copy
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