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http://www.addgene.org/11987

Species: Homo sapiens
Genetic Insert: Signal transducer and activator of transcription 1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6000; Vector Backbone:pcDNA3-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note hSTAT1 in this plasmid contains the mutations A188T and Q718R compared to GenBank BC002704. The impact of these mutations on plasmid functionality is unknown.

Proper citation: RRID:Addgene_11987 Copy   

•   Source: Addgene
  

http://www.addgene.org/11989

Species: Homo sapiens
Genetic Insert: MMP7
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6000; Vector Backbone:pcDNA3-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_11989 Copy   

•   Source: Addgene
  

http://www.addgene.org/119863

Vector Backbone Description: Backbone Size:7298; Vector Backbone:pCIG3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31594807
Comments: Please note that though there are some discrepancies between the backbone sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as described.

Proper citation: RRID:Addgene_119863 Copy   

•   Source: Addgene
  

http://www.addgene.org/119860

Species: Escherichia coli str. K-12
Genetic Insert: outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
Vector Backbone Description: Vector Backbone:pET503; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30275279

Proper citation: RRID:Addgene_119860 Copy   

•   Source: Addgene
  

http://www.addgene.org/119906

Species: Synthetic
Genetic Insert: CymRV5-nls-GFP
Vector Backbone Description: Backbone Size:5152; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK. Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119906 Copy   

•   Source: Addgene
  

http://www.addgene.org/119903

Species: Synthetic
Genetic Insert: cuOx144 array
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pDEST-Bxb1attB-loxP-PGK-puro-loxP; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please note there are a few ambiguous bases in Addgene's NGS result. This is due to NGS having difficulty assembling repetitive sequences. The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation. Two separate sequencing results shown above illustrate variable number of repeats. Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119903 Copy   

•   Source: Addgene
  

http://www.addgene.org/119867

Species: Danio rerio
Genetic Insert: myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3121; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358

Proper citation: RRID:Addgene_119867 Copy   

•   Source: Addgene
  

http://www.addgene.org/119900

Species: Mus musculus
Genetic Insert: ret
Vector Backbone Description: Vector Backbone:pBS; Vector Types:in situ hybridization probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20064391
Comments: This plasmid can be used to generate an in situ hybridization antisense probe for mouse C-ret. Linearize the plasmid with NotI and use T7 to transcribe the ~1kb probe.

Proper citation: RRID:Addgene_119900 Copy   

•   Source: Addgene
  

http://www.addgene.org/119868

Species: Danio rerio
Genetic Insert: NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3008; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358

Proper citation: RRID:Addgene_119868 Copy   

•   Source: Addgene
  

http://www.addgene.org/119901

Species: Mycobacteriophage Bxb1
Genetic Insert: Mammalian codon optimized Bxb1 integrase
Vector Backbone Description: Backbone Size:4905; Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119901 Copy   

•   Source: Addgene
  

http://www.addgene.org/119908

Species: Synthetic
Genetic Insert: HA-tdMS2cp-tagRFP-Tx2-nls
Vector Backbone Description: Backbone Size:3526; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119908 Copy   

•   Source: Addgene
  

http://www.addgene.org/119909

Species: Synthetic
Genetic Insert: TetRFlag-nls-tdTom
Vector Backbone Description: Backbone Size:5670; Vector Backbone:ePiggyBac-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK. Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_119909 Copy   

•   Source: Addgene
  

http://www.addgene.org/119855

Genetic Insert: dCas13b-GGS-NYTHDF1
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280

Proper citation: RRID:Addgene_119855 Copy   

•   Source: Addgene
  

http://www.addgene.org/119853

Species: Escherichia coli (strain K12)
Genetic Insert: precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
Vector Backbone Description: Vector Backbone:pBR322 with T7 promoter; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30275279
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_119853 Copy   

•   Source: Addgene
  

http://www.addgene.org/119858

Genetic Insert: dCas13b-GGS-Sun
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280

Proper citation: RRID:Addgene_119858 Copy   

•   Source: Addgene
  

http://www.addgene.org/119856

Genetic Insert: dCas13b-GGS-NYTHDF2(100-200)
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280

Proper citation: RRID:Addgene_119856 Copy   

•   Source: Addgene
  

http://www.addgene.org/119857

Genetic Insert: dCas13b-GGS-NYTHDF1(100-200)
Vector Backbone Description: Vector Backbone:cmv d0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30183280

Proper citation: RRID:Addgene_119857 Copy   

•   Source: Addgene
  

http://www.addgene.org/119843

Species: Mus musculus
Genetic Insert: Type I procollagen α1 chain
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287488
Comments: These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]).

Proper citation: RRID:Addgene_119843 Copy   

•   Source: Addgene
  

http://www.addgene.org/119844

Species: Mus musculus
Genetic Insert: Type I procollagen α1 chain
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287488
Comments: These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]).

Proper citation: RRID:Addgene_119844 Copy   

•   Source: Addgene
  

http://www.addgene.org/119842

Species: Mus musculus
Genetic Insert: Type I procollagen α1 chain
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287488
Comments: These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]).

Proper citation: RRID:Addgene_119842 Copy   

•   Source: Addgene