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Species: Synthetic
Genetic Insert: BCD 0.45K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200055 Copy
Species: Synthetic
Genetic Insert: BCD 0.2K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200056 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tRNA-gRNA scaffold
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37326023
Comments: A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
Proper citation: RRID:Addgene_200227 Copy
Species: Sapria himilayana
Genetic Insert: N-terminal transit peptide from putative cytosolic GlyRS
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39693336
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.08.01.551541v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_202648 Copy
Genetic Insert: FAST marker
Vector Backbone Description: Vector Backbone:pJOG272; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:36978193
Proper citation: RRID:Addgene_202775 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cas9 (codon optimized for human)
Vector Backbone Description: Backbone Size:4898; Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37326023
Proper citation: RRID:Addgene_192603 Copy
Genetic Insert: LV 3CL protease
Vector Backbone Description: Vector Backbone:pAG426GAL-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39762652
Proper citation: RRID:Addgene_203507 Copy
Species: Sapria himilayana
Genetic Insert: N-terminal transit peptide from putative cytosolic MetRS
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39693336
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.08.01.551541v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_202650 Copy
Species: Sapria himilayana
Genetic Insert: N-terminal transit peptide from putative cytosolic ThrRS
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39693336
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.08.01.551541v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_202652 Copy
Species: Sapria himilayana
Genetic Insert: N-terminal transit peptide from putative cytosolic ProRS
Vector Backbone Description: Vector Backbone:pK7FWG2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39693336
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.08.01.551541v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_202651 Copy
Genetic Insert: SHV 3CL protease
Vector Backbone Description: Vector Backbone:pAG416GAL10-ccdB-6stop; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39762652
Proper citation: RRID:Addgene_203473 Copy
Species: Spizellomyces punctatus
Genetic Insert: SpuFz1 omegaRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37380027
Proper citation: RRID:Addgene_205261 Copy
Species: Spizellomyces punctatus
Genetic Insert: SpuFz1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37380027
Proper citation: RRID:Addgene_205260 Copy
Species: Synthetic
Genetic Insert: Pong TPase LA T2A ORF1SC1 ONE
Vector Backbone Description: Backbone Marker:ABRC; Backbone Size:11648; Vector Backbone:pEarleyGate 100; Vector Types:Plant Expression, Binary; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37273575
Proper citation: RRID:Addgene_196137 Copy
Species: Homo sapiens
Genetic Insert: ARL1
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pET3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_201866 Copy
Species: Homo sapiens
Genetic Insert: CYB5R3
Vector Backbone Description: Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36543799
Proper citation: RRID:Addgene_204570 Copy
Species: Homo sapiens
Genetic Insert: CDK5RAP3
Vector Backbone Description: Vector Backbone:phAG-MCL; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36543799
Proper citation: RRID:Addgene_204571 Copy
Species: Homo sapiens
Genetic Insert: α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25544608
Proper citation: RRID:Addgene_198245 Copy
Species: Homo sapiens
Genetic Insert: ARL9
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pET3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_201873 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
Vector Backbone Description: Vector Backbone:pHT43; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37348112
Comments: Plasmid can also be transformed in Bacillus subtilis, grown at 37 C
Proper citation: RRID:Addgene_203813 Copy
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