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Species: Mus musculus
Genetic Insert: SNX19-PX (528-664)
Vector Backbone Description: Vector Backbone:pGEX4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30948714
Comments: Amino Acid Sequence: SPDGPVVIQNLRITGTITAREHSGTGFHPYTLYTVKYETVLNGENSSGLQQLAYHTVNRRYREFLNLQTRLEEKPDLRKFIKNVKGPKKLFPDLPFGNMDSDRVEARKSLLESFLKQLCAIPEIGNSEEVQEFLALN
Proper citation: RRID:Addgene_119100 Copy
Species: Danio rerio
Genetic Insert: U6a:2xCom-sgRNA
Vector Backbone Description: Backbone Size:3565; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119077 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled1-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence AGCGCCTACGCGCATGGCCT.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119070 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled2-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence GGCCACAGGCGATTATCGCC.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119071 Copy
Species: Homo sapiens
Genetic Insert: NAD(P)H dehydrogenase, quinone 1 (NQO1)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15809436
Proper citation: RRID:Addgene_119058 Copy
Genetic Insert: HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Vector Backbone Description: Backbone Size:2995; Vector Backbone:pGEM-T-Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119065 Copy
Genetic Insert: sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
Vector Backbone Description: Vector Backbone:YTK095; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995532
Proper citation: RRID:Addgene_119157 Copy
Genetic Insert: sgRNA NT1/RR1 (rfp)
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119268 Copy
Genetic Insert: B. subtilis deltaTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Comments:
IMPORT NOTES: Special character found in field(Gene or insert Name)
Proper citation: RRID:Addgene_119266 Copy
Species: bacteriophage P1
Genetic Insert: cre
Vector Backbone Description: Backbone Size:6600; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10334853
Comments: pBS513 carries the wt cre gene under the control of the elongation factor-1 alpha (EF-1 alpha) promoter. The construct carries an upstream Kozak modification to increase expression. The resulting construct provides very strong expression in many different types of cells, including murine embryonic stem (ES) cells. For cell culture work this construct is recommended as a replacement for the CMV-cre construct pBS185 as it provides much stronger expression. However, pBS513 is not suitable for use in transgenic mice as such EF-1 alpha transgene constructs are often inactive in many tissues.
Please note: The following enzymes are NOT single cutters: PstI, AflIII, and SacI. We do not suggest using these enzymes for cloning.
Proper citation: RRID:Addgene_11918 Copy
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119270 Copy
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119271 Copy
Genetic Insert: sgRNA NT1/RR1 (rfp)
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119258 Copy
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119255 Copy
Genetic Insert: sgRNA folA (P. aeruginosa)
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119263 Copy
Genetic Insert: STOP
Vector Backbone Description: Backbone Size:3806; Vector Backbone:n/a; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8231893
Comments: pBS302 carries two directly repeated loxP sites flanking a synthetic DNA sequence designated "STOP." The lox-square STOP cassette sits on a NotI fragment that can excised and gel-purified for injection into fertilized zygotes. The STOP sequence is designed to thwart productive expression of a downstream gene under the control of an upstream promoter (to be inserted in the SfiI-SpeI polylinker region). It will have been removed in cells expressing Cre, or in descendants of cells that previously had expressed Cre, because of Cre-mediated recombination at the loxP sites. The STOP sequence is the same as used to regulate T-Ag expression in pBS241.
Proper citation: RRID:Addgene_11925 Copy
Genetic Insert: B. subtilis attTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119264 Copy
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119261 Copy
Species: bacteriophage P1
Genetic Insert: EGFP-cre
Vector Backbone Description: Backbone Size:6600; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10334853
Comments: pBS598 carries the EGFPcre fusion gene under the control of the strong elongation factor EF1alpha promoter. This promoter expresses at high levels in most cell types, including embryonic stem (ES) cells. However its use in transgenic mice is not recommended: the promoter is often silenced in a transgene setting. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression.
Note: pBS513 (EF1alpha-cre) is very similar to pBS598 but carries the wt cre gene and thus serves as the non-fluorescent counterpart of pBS598.
Proper citation: RRID:Addgene_11923 Copy
Genetic Insert: sgRNA phzM (P. aeruginosa)
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119247 Copy
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